Amano A, Kuboniwa M, Nakagawa I, Akiyama S, Morisaki I, Hamada S
Division of Special Care Dentistry, Osaka University Faculty of Dentistry, Suita, Japan.
J Dent Res. 2000 Sep;79(9):1664-8. doi: 10.1177/00220345000790090501.
Porphyromonas gingivalis fimA gene encoding fimbrillin, a subunit of fimbriae, has been classified into 5 genotypes (types I to V) based on their nucleotide sequences. Here, we investigated the relationship between the prevalence of these fimA genotypes and periodontal health status in adults. Dental plaque specimens obtained from 380 periodontally healthy adults and 139 periodontitis patients were analyzed by the PCR method. P. gingivalis was detected in 36.8% of the healthy subjects and in 87.1% of the periodontitis patients. Among the P. gingivalis-positive healthy adults, the most prevalent fimA type was type I (76.1%), followed by type V. In contrast, a majority of the periodontitis patients carried type II fimA organisms (66.1%), followed by type IV. The univariate analysis illustrated that periodontitis was associated with the occurrences of type I fimA (OR 0.16), type II (OR 44.44), type III (1.96), type IV (13.87), and type V (1.40). These findings clearly indicate that there are both disease-associated and non-disease-associated strains of P. gingivalis, and that their infectious traits influencing periodontal health status could be differentiated based on the clonal variation of fimA genes.
牙龈卟啉单胞菌的fimA基因编码菌毛蛋白（菌毛的一个亚基），根据其核苷酸序列已被分为5种基因型（I型至V型）。在此，我们研究了这些fimA基因型的流行情况与成年人牙周健康状况之间的关系。通过聚合酶链反应（PCR）方法分析了从380名牙周健康成年人和139名牙周炎患者获取的牙菌斑标本。在36.8%的健康受试者和87.1%的牙周炎患者中检测到牙龈卟啉单胞菌。在牙龈卟啉单胞菌阳性的健康成年人中，最常见的fimA类型是I型（76.1%），其次是V型。相比之下，大多数牙周炎患者携带II型fimA菌（66.1%），其次是IV型。单因素分析表明，牙周炎与I型fimA（比值比0.16）、II型（比值比44.44）、III型（1.96）、IV型（13.87）和V型（1.40）的出现有关。这些发现清楚地表明，存在与疾病相关和与疾病无关的牙龈卟啉单胞菌菌株，并且可以根据fimA基因的克隆变异来区分它们影响牙周健康状况的感染特性。
