Liu Chenglan, Xu Wenna, Liu Fengmao, Jiang Shuren
Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, 100094, PR China.
Mycopathologia. 2007 Sep;164(3):127-34. doi: 10.1007/s11046-007-9017-8. Epub 2007 Jul 13.
The present work deals with the capability for producing fumonisin by Fusarium proliferatum strains isolated from asparagus in China. Fifty of F. proliferatum strains were randomly selected and incubated on cultures of maize grain and asparagus spear, respectively. Fumonisin levels (FB(1) and FB(2)) were determined by high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). The results showed that all 50 strains produced fumonisins in maize culture within a wide range of concentrations, 10-11,499 microg/g and 2-6,598 microg/g for FB(1) and FB(2), respectively. On culture of asparagus spear,48 strains (96%) produced fumonisins in the range 0.2-781.6 microg/g and no detected to 40.3 microg/g for FB(1) and FB(2), respectively. All of F. proliferatum strains produced much higher levels of FB(1), FB(2) and total fumonisins (FB(1 )+ FB(2)) in maize grain culture than in asparagus spear culture. Meanwhile, fumonisin B(3) (FB(3)) was identified in all maize culture extracts and most of asparagus spear culture extracts. This is the first study carried out the fumonisin-producing ability of F. proliferatum strains isolated from asparagus in China. The information obtained is useful for assessing the risk of fumonisins contamination in asparagus spear.
本研究探讨了从中国芦笋中分离出的层出镰刀菌菌株产生伏马毒素的能力。随机选取50株层出镰刀菌菌株，分别在玉米粒和芦笋茎培养基上培养。伏马毒素水平（FB(1)和FB(2)）通过高效液相色谱-电喷雾电离串联质谱法（HPLC-ESI-MS/MS）测定。结果表明，所有50株菌株在玉米培养基中均产生伏马毒素，浓度范围较广，FB(1)和FB(2)分别为10 - 11499μg/g和2 - 6598μg/g。在芦笋茎培养基上，48株（96%）菌株产生伏马毒素，FB(1)和FB(2)的浓度范围分别为0.2 - 781.6μg/g和未检出至40.3μg/g。所有层出镰刀菌菌株在玉米粒培养基中产生的FB(1)、FB(2)和总伏马毒素（FB(1)+FB(2)）水平均远高于芦笋茎培养基。同时，在所有玉米培养基提取物和大多数芦笋茎培养基提取物中均鉴定出伏马毒素B(3)（FB(3)）。这是首次对从中国芦笋中分离出的层出镰刀菌菌株产生伏马毒素的能力进行的研究。所获得的信息有助于评估芦笋茎中伏马毒素污染的风险。
