Departamento de Microbiología e Inmunología and §Departamento de Química, Facultad de Ciencias Exactas Fco-Qcas y Naturales, Universidad Nacional de Rio Cuarto, Ruta 36 Km 601, 5800 Río Cuarto, Córdoba, Argentina.
J Agric Food Chem. 2011 Nov 23;59(22):12264-9. doi: 10.1021/jf204035y. Epub 2011 Oct 26.
A survey was carried out to determine Fusarium species and fumonisin contamination in 55 durum wheat (Triticum turgidum L. var. durum) samples collected during two harvest seasons (2007 and 2008) using HPLC and further LC-MS/MS confirmation. All samples showed Fusarium contamination with infection levels ranging from 8 to 66%, F. proliferatum being the species most frequently isolated during 2007 and the second most frequently isolated one during the 2008 harvest season, respectively. Natural contamination with fumonisins was found in both harvest seasons. In 2007, 97% of the samples showed total fumonisin (FB(1) + FB(2)) levels ranging from 10.5 to 1245.7 ng/g, while very low levels of fumonisins were detected in samples collected during 2008. These results could be explained by differences in the amount of rainfall during both periods evaluated. A selected number (n = 48) of F. proliferatum isolates showed fumonisin production capability on autoclaved rice. This is the first report of the presence of natural fumonisins in durum wheat grains.
进行了一项调查，以使用 HPLC 并进一步通过 LC-MS/MS 确认，来确定 55 个硬粒小麦（Triticum turgidum L. var. durum）样品在两个收获季节（2007 年和 2008 年）中镰刀菌物种和伏马菌素污染情况。所有样品均显示出镰刀菌污染，感染水平在 8%至 66%之间，2007 年最常分离到的物种是层出镰刀菌，而在 2008 年收获季节中是第二常分离到的物种。两个收获季节均发现了天然伏马菌素污染。2007 年，97%的样品中总伏马菌素（FB(1) + FB(2)）水平范围为 10.5 至 1245.7ng/g，而在 2008 年采集的样品中检测到的伏马菌素含量非常低。这些结果可以用评估期间降雨量的差异来解释。对数量有限的（n = 48）层出镰刀菌分离株在高压灭菌大米上显示出伏马菌素的产生能力。这是首次在硬粒小麦谷物中发现天然伏马菌素。
