脉冲场电泳中DNA迁移的袋模型。
Bag model for DNA migration during pulsed-field electrophoresis.
作者信息
Chu G
机构信息
Department of Medicine, Stanford University School of Medicine, CA 94305.
出版信息
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11071-5. doi: 10.1073/pnas.88.24.11071.
A model for pulsed-field electrophoresis was developed by picturing large DNA as a deformable "bag" that (i) moves with limiting mobility in a continuous electric field, (ii) adopts an orientation aligned with the field direction, and (iii) reorients after a change in field direction in a size-dependent manner. The model correctly predicted the resolution of large DNA in a pulsed field including the surprising phenomena of mobility inversion, lateral band spreading, and improved resolution for obtuse angles. A simple parametrization agreed with observations of two completely different aspects of DNA behavior: bulk mobility as measured during gel electrophoresis and molecular reorientation as measured by linear dichroism. The model also provides quantitative guidelines for setting experimental parameters in pulsed-field electrophoresis experiments.
通过将大型DNA描绘成一个可变形的“袋子”，建立了一种脉冲场电泳模型，该“袋子”具有以下特点：（i）在连续电场中以有限的迁移率移动；（ii）采取与场方向一致的取向；（iii）在场方向改变后以大小依赖的方式重新取向。该模型正确地预测了脉冲场中大型DNA的分辨率，包括迁移率反转、横向条带扩展以及钝角时分辨率提高等惊人现象。一个简单的参数化与DNA行为两个完全不同方面的观察结果相符：凝胶电泳过程中测量的整体迁移率以及线性二色性测量的分子重新取向。该模型还为脉冲场电泳实验中设置实验参数提供了定量指导。
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