Oana H, Doi M, Ueda M, Yoshikawa K
School of Engineering, Nagoya University, Japan.
Electrophoresis. 1997 Oct;18(11):1912-5. doi: 10.1002/elps.1150181104.
Recently, we found that, in concentrated neutral solutions, DNA molecules migrate in linear conformation under steady electric field. In this paper, we report the conformational change of DNA during 120 degree crossed-field electrophoresis in the same polymer solution. We found that, in concentrated polyacrylamide solutions, the reorientation process of DNAs becomes simple: the DNA goes back along the previous track and the reorientation time is longer for larger DNA. Such a backtrack motion has been thought to be an essential motion for the separation of DNA fragments in pulsed field gel electrophoresis. We expect that this phenomenon is useful for a more efficient separation technique of large DNAs than the current pulsed field gel electrophoresis.
最近，我们发现，在浓缩中性溶液中，DNA分子在稳定电场下以线性构象迁移。在本文中，我们报告了在相同聚合物溶液中进行120度交变电场电泳时DNA的构象变化。我们发现，在浓缩聚丙烯酰胺溶液中，DNA的重新定向过程变得简单：DNA沿先前轨迹返回，较大的DNA重新定向时间更长。这种回溯运动被认为是脉冲场凝胶电泳中DNA片段分离的基本运动。我们期望这种现象对于一种比当前脉冲场凝胶电泳更高效的大DNA分离技术是有用的。
