Lalande M, Noolandi J, Turmel C, Rousseau J, Slater G W
National Research Council Canada, Biotechnology Research Institute, Montréal, Québec.
Proc Natl Acad Sci U S A. 1987 Nov;84(22):8011-5. doi: 10.1073/pnas.84.22.8011.
The biased reptation theory has been applied to the pulsed-field electrophoresis of DNA in agarose gels. A computer simulation of the theoretical model that calculates the mobility of large DNA molecules as a function of agarose pore size, DNA chain properties, and electric field conditions has been used to generate mobility curves for DNA molecules in the size range of the larger yeast chromosomes. Pulsed-field electrophoresis experiments resulting in the establishment of an electrophoretic karyotype for yeast, where the mobility of the DNA fragments is a monotonic function of molecular size for the entire size range that is resolved (200-2200 kilobase pairs), has been compared to the theoretical mobility curves generated by the computer model. The various physical mechanisms and experimental conditions responsible for band inversion and improved electrophoretic separation are identified and discussed in the framework of the model.
有偏蠕动理论已应用于琼脂糖凝胶中DNA的脉冲场电泳。一个理论模型的计算机模拟，该模型计算大DNA分子的迁移率作为琼脂糖孔径、DNA链特性和电场条件的函数，已被用于生成较大酵母染色体大小范围内DNA分子的迁移率曲线。脉冲场电泳实验建立了酵母的电泳核型，其中DNA片段的迁移率是整个可分辨大小范围（200 - 2200千碱基对）内分子大小的单调函数，已与计算机模型生成的理论迁移率曲线进行了比较。在该模型的框架内，确定并讨论了导致条带反转和改善电泳分离的各种物理机制和实验条件。
