Kwaaitaal Mark A C J, de Vries Sacco C
Laboratory of Biochemistry, Wageningen University, Dreijenlaan 3, 6703 HA Wageningen, the Netherlands.
J Exp Bot. 2007;58(11):2887-96. doi: 10.1093/jxb/erm103. Epub 2007 Jul 13.
The SOMATIC EMBRYOGENESIS RECEPTOR KINASE 1 (SERK1) gene is expressed in the procambium of the vascular bundles in roots, hypocotyls, and inflorescence stems. In younger parts of roots and hypocotyls, SERK1 expression was less restricted and was also observed in protoxylem cells, immature metaxylem cells and phloem companion cells. In roots, SERK1 expression was first detected in root vascular stem cells and was notably absent from the QC. In general, the SERK1 protein level as visualized by expression of a SERK1-YFP fusion protein closely followed the pattern of gene expression. In hypocotyls, prolonged application of 2,4-D resulted in extensive unorganized proliferation of SERK1 expressing cells originating from the procambium and pericycle. In roots, 2,4-D treatment results in an increase in SERK1 transcription that results in a moderate increase in the amount of SERK1-YFP fusion protein. The restricted vascular pattern of SERK1 expression in roots remains unaffected after 2,4-D treatment.
体细胞胚胎发生受体激酶1(SERK1)基因在根、下胚轴和花序茎的维管束原形成层中表达。在根和下胚轴较幼嫩的部分,SERK1的表达限制较少,在原生木质部细胞、未成熟后生木质部细胞和韧皮部伴细胞中也有观察到。在根中,SERK1的表达首先在根维管干细胞中检测到,而在静止中心明显缺失。总体而言,通过SERK1-YFP融合蛋白表达可视化的SERK1蛋白水平与基因表达模式密切相关。在下胚轴中,长时间施用2,4-D导致源自原形成层和中柱鞘的SERK1表达细胞广泛无序增殖。在根中,2,4-D处理导致SERK1转录增加,从而使SERK1-YFP融合蛋白的量适度增加。2,4-D处理后,根中SERK1表达的受限维管模式仍未受影响。