Li Dong, Wang Guo-Yun, Dong Bai-Hua, Zhang Yue-Cun, Wang Yuan-Xiu, Sun Bing-Cui
Department of Biological Technology, School of Chemistry and Chemical Engineering, Jinan University, Jinan, China.
Cells Tissues Organs. 2007;186(3):169-79. doi: 10.1159/000105674. Epub 2007 Jul 12.
The placenta is an attractive new source of mesenchymal stem cells (MSCs), but the biological characteristics of placenta-derived MSCs (P-MSCs) have not yet been characterized. We successfully isolated, cultured and expanded P-MSCs using routine methods. Under appropriate induction conditions, these cells can differentiate into bone, cartilage, fat and hepatocyte-like cells. In addition, the proliferative response of P-MSCs to different cytokines was monitored using the MTT assay. The results show that low concentrations of proinflammatory cytokines, e.g. RANTES, interleukin (IL)-1, IL-6 and IL-8 can stimulate the proliferation of P-MSCs in a dose-dependent manner, peaking at concentrations of 40 ng/ml of RANTES, 10 ng/ml of IL-1 and IL-6, and 150 ng/ml of IL-8 (p < 0.01). The level of proliferation decreased when the concentration of these four cytokines increased beyond these values. On the other hand, anti-inflammatory cytokines hepatocyte growth factor and IL-4 had an inhibitory effect on P-MSCs. In conclusion, the placenta contains MSCs that are consistent with the characteristics of bone marrow MSCs. Low concentrations of proinflammatory chemokines stimulated the proliferation of P-MSCs while anti-inflammatory cytokines inhibited the growth of P-MSCs in a dose-dependent manner.
胎盘是间充质干细胞（MSCs）一个有吸引力的新来源，但胎盘来源的间充质干细胞（P-MSCs）的生物学特性尚未得到明确。我们使用常规方法成功分离、培养并扩增了P-MSCs。在适当的诱导条件下，这些细胞可分化为骨、软骨、脂肪和肝细胞样细胞。此外，使用MTT法监测P-MSCs对不同细胞因子的增殖反应。结果表明，低浓度的促炎细胞因子，如RANTES、白细胞介素（IL）-1、IL-6和IL-8可呈剂量依赖性刺激P-MSCs的增殖，在RANTES浓度为40 ng/ml、IL-1和IL-6浓度为10 ng/ml、IL-8浓度为150 ng/ml时达到峰值（p < 0.01）。当这四种细胞因子的浓度超过这些值时，增殖水平下降。另一方面，抗炎细胞因子肝细胞生长因子和IL-4对P-MSCs有抑制作用。总之，胎盘含有与骨髓间充质干细胞特性一致的间充质干细胞。低浓度的促炎趋化因子刺激P-MSCs的增殖，而抗炎细胞因子则呈剂量依赖性抑制P-MSCs的生长。
