Nowwarote Nunthawan, Sukarawan Waleerat, Kanjana Kiattipan, Pavasant Prasit, Fournier Benjamin P J, Osathanon Thanaphum
Excellence Center in Regenerative Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
Department of Pediatric Dentistry, Faculty of Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok 10330, Thailand.
R Soc Open Sci. 2018 Oct 31;5(10):180864. doi: 10.1098/rsos.180864. eCollection 2018 Oct.
Interleukin 6 (IL-6) plays various roles including stem cell regulation. The present study investigated the effect of IL-6 on cell proliferation, colony forming unit ability, stem cell marker expression and differentiation ability in stem cells isolated from human exfoliated deciduous teeth (SHEDs). We reported that the isolated cells from dental pulp tissues for deciduous teeth expressed CD44, CD90 and CD105 but not CD45. These cells were able to differentiate into osteoblasts, adipocytes and neuronal-like cells. IL-6 treatment resulted in the significant increase of and mRNA expression. However, IL-6 had no effect on cell proliferation and colony forming unit ability. IL-6 did not alter adipogenic and neurogenic differentiation potency. IL-6 supplementation in osteogenic medium led to a significant increase of mineralization. Furthermore, IL-6 upregulated and mRNA levels. In conclusion, IL-6 participates in the regulation of pluripotent marker expression and is also involved in mineralization process of SHEDs. Hence, IL-6 could be employed as a supplementary substance in culture medium to maintain stemness and to induce osteogenic induction in SHEDs for future regenerative cell therapy.
白细胞介素6（IL-6）发挥着包括干细胞调节在内的多种作用。本研究调查了IL-6对从人乳牙（SHEDs）分离的干细胞的细胞增殖、集落形成单位能力、干细胞标志物表达及分化能力的影响。我们报告称，从乳牙牙髓组织分离的细胞表达CD44、CD90和CD105，但不表达CD45。这些细胞能够分化为成骨细胞、脂肪细胞和神经元样细胞。IL-6处理导致 和 mRNA表达显著增加。然而，IL-6对细胞增殖和集落形成单位能力没有影响。IL-6未改变成脂和成神经分化潜能。在成骨培养基中添加IL-6导致矿化显著增加。此外，IL-6上调了 和 mRNA水平。总之，IL-6参与多能标志物表达的调节，也参与SHEDs的矿化过程。因此，IL-6可作为培养基中的补充物质，以维持SHEDs的干性并诱导其成骨诱导，用于未来的再生细胞治疗。
