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有丝分裂期间泛素激活酶与HeLa细胞染色体的关联

Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis.

作者信息

Cook J C, Chock P B

机构信息

Section of Metabolic Regulation, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892.

出版信息

Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11388-92. doi: 10.1073/pnas.88.24.11388.

DOI:10.1073/pnas.88.24.11388
PMID:1763053
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC53140/
Abstract

Ubiquitin-activating enzyme (E1) is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. Antibodies raised against E1 were affinity purified and used for immunostaining HeLa cells. Condensed chromosomes in mitotic cells were found to be strongly immunoreactive. Chromosomes from metaphase-arrested HeLa cells were isolated and chromosome-associated proteins were analyzed by Western blotting. E1 was detected in fractions containing isolated chromosomes. These results suggest that E1 is associated with condensed chromosomes during mitosis.

摘要

泛素激活酶（E1）是通向泛素-蛋白质缀合物形成途径中的第一种酶。针对E1产生的抗体经亲和纯化后用于对HeLa细胞进行免疫染色。发现有丝分裂细胞中的浓缩染色体具有强烈的免疫反应性。分离出处于中期停滞的HeLa细胞的染色体，并通过蛋白质印迹法分析与染色体相关的蛋白质。在含有分离出的染色体的组分中检测到了E1。这些结果表明，E1在有丝分裂期间与浓缩染色体相关联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/bddbc28fe7df/pnas01074-0420-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/e50d9d98c582/pnas01074-0418-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/86e2a7e06df6/pnas01074-0419-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/0801a54e1d4a/pnas01074-0419-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/fdb302a44122/pnas01074-0420-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/bddbc28fe7df/pnas01074-0420-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/e50d9d98c582/pnas01074-0418-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/86e2a7e06df6/pnas01074-0419-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/0801a54e1d4a/pnas01074-0419-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/fdb302a44122/pnas01074-0420-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8cc/53140/bddbc28fe7df/pnas01074-0420-b.jpg

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引用本文的文献

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Nucleic Acids Res. 2012 Nov 1;40(20):10187-202. doi: 10.1093/nar/gks820. Epub 2012 Aug 31.
2
Purification and characterization of a ubiquitin carrier protein kinase from HeLa cells.从人宫颈癌HeLa细胞中纯化及鉴定泛素载体蛋白激酶
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本文引用的文献

1
Evidence for the involvement of H1 histone phosphorylation in chromosome condensation.H1组蛋白磷酸化参与染色体凝聚的证据。
Nature. 1980 Mar 13;284(5752):181-3. doi: 10.1038/284181a0.
2
A mouse temperature-sensitive mutant defective in H1 histone phosphorylation is defective in deoxyribonucleic acid synthesis and chromosome condensation.一种在H1组蛋白磷酸化方面存在缺陷的小鼠温度敏感突变体在脱氧核糖核酸合成和染色体凝聚方面存在缺陷。
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Intracellular localization of human DNA polymerase alpha with monoclonal antibodies.
利用单克隆抗体对人DNA聚合酶α进行细胞内定位
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Intracellular localization of DNA polymerase alpha.DNA聚合酶α的细胞内定位
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Production of reagent antibodies.试剂抗体的生产。
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Changes in distribution of nuclear matrix antigens during the mitotic cell cycle.有丝分裂细胞周期中核基质抗原分布的变化。
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Thermolability of ubiquitin-activating enzyme from the mammalian cell cycle mutant ts85.来自哺乳动物细胞周期突变体ts85的泛素激活酶的热稳定性
Cell. 1984 May;37(1):43-55. doi: 10.1016/0092-8674(84)90299-x.
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"Covalent affinity" purification of ubiquitin-activating enzyme.泛素激活酶的“共价亲和”纯化
J Biol Chem. 1982 Mar 10;257(5):2537-42.
9
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.“蛋白质免疫印迹法”：蛋白质从十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳转移至未修饰的硝酸纤维素膜上，并用抗体和放射性碘化蛋白A进行放射自显影检测。
Anal Biochem. 1981 Apr;112(2):195-203. doi: 10.1016/0003-2697(81)90281-5.
10
Metabolism of ubiquitinated histones.泛素化组蛋白的代谢
J Biol Chem. 1981 Jun 10;256(11):5916-20.