有丝分裂期间泛素激活酶与HeLa细胞染色体的关联
Association of ubiquitin-activating enzyme with HeLa cell chromosomes during mitosis.
作者信息
Cook J C, Chock P B
机构信息
Section of Metabolic Regulation, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
出版信息
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11388-92. doi: 10.1073/pnas.88.24.11388.
Ubiquitin-activating enzyme (E1) is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. Antibodies raised against E1 were affinity purified and used for immunostaining HeLa cells. Condensed chromosomes in mitotic cells were found to be strongly immunoreactive. Chromosomes from metaphase-arrested HeLa cells were isolated and chromosome-associated proteins were analyzed by Western blotting. E1 was detected in fractions containing isolated chromosomes. These results suggest that E1 is associated with condensed chromosomes during mitosis.
泛素激活酶（E1）是通向泛素-蛋白质缀合物形成途径中的第一种酶。针对E1产生的抗体经亲和纯化后用于对HeLa细胞进行免疫染色。发现有丝分裂细胞中的浓缩染色体具有强烈的免疫反应性。分离出处于中期停滞的HeLa细胞的染色体，并通过蛋白质印迹法分析与染色体相关的蛋白质。在含有分离出的染色体的组分中检测到了E1。这些结果表明，E1在有丝分裂期间与浓缩染色体相关联。
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