Cook J C, Chock P B
Section on Metabolic Regulation, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.
Biochem Biophys Res Commun. 1991 Jan 31;174(2):564-71. doi: 10.1016/0006-291x(91)91454-k.
Ubiquitin-activating enzyme, "E1", is the first enzyme in the pathway leading to formation of ubiquitin-protein conjugates. We present immunocytochemical evidence that Ubiquitin-activating enzyme is concentrated in the cell nucleus. This finding points to the nucleus as the major site of action of this enzyme. Since ubiquitin itself is not similarly compartmentalized, this result suggests a high level of ubiquitin conjugate formation in the nucleus with a rapid turnover of ubiquitin conjugates.
泛素激活酶“E1”是导致泛素-蛋白质缀合物形成途径中的首个酶。我们提供免疫细胞化学证据表明泛素激活酶集中于细胞核中。这一发现表明细胞核是该酶的主要作用位点。由于泛素本身并无类似的区室化分布，这一结果提示细胞核中存在高水平的泛素缀合物形成，且泛素缀合物周转迅速。
