Gao Li, Yu Shuhong, Wu Yuefeng, Shan Baoen
College of Life Science, Hebei Normal University, Shijiazhuang, Hebei Province, People's Republic of China.
DNA Cell Biol. 2007 Jul;26(7):485-9. doi: 10.1089/dna.2007.0579.
The purpose of this study was to examine the effects of antitumor activity of the venom from the spider Macrothele raven (Araneae, Hexathelidae) on the human breast carcinoma cell line, MCF-7. The spider venom affected cell viability in a dose- and time-dependent manner as observed by [(3)H]-methyl thymidine incorporation assay. Cytotoxicity changes in MCF-7 cells caused by the spider venom at concentrations of 10, 20, and 40 mug/mL were determined by lactate dehydrogenase release assay. Flow cytometry showed that the spider venom induced apoptosis and necrosis of MCF-7 cells at these concentrations. MCF-7 cells treated with spider venom were accumulated on the G(2)/M and G(0)/G(1) phases. In addition, Western blotting analysis indicated that one of the pharmacological mechanisms of spider venom was to activate the expression of p21. In vivo examination of the inhibition of tumor growth in nude mice by the spider venom (at concentrations of 1.6, 1.8, and 2.0 mug/g mice) revealed that tumor size significantly decreased compared to controls by 21 days of treatment and at all points of analysis thereafter for 7 weeks (p < 0.01). We thus propose that the in vivo and in vitro effects of the spider venom can be possibly estimated.
本研究旨在考察雷氏大疣蛛（蜘蛛目，六疣蛛科）毒液对人乳腺癌细胞系MCF-7的抗肿瘤活性作用。通过[³H]-甲基胸腺嘧啶核苷掺入试验观察到，蜘蛛毒液以剂量和时间依赖性方式影响细胞活力。采用乳酸脱氢酶释放试验测定了浓度为10、20和40μg/mL的蜘蛛毒液对MCF-7细胞的细胞毒性变化。流式细胞术显示，在这些浓度下，蜘蛛毒液诱导MCF-7细胞凋亡和坏死。用蜘蛛毒液处理的MCF-7细胞积聚在G₂/M期和G₀/G₁期。此外，蛋白质免疫印迹分析表明，蜘蛛毒液的药理机制之一是激活p21的表达。对蜘蛛毒液（浓度为1.6、1.8和2.0μg/g小鼠）抑制裸鼠肿瘤生长的体内研究表明，与对照组相比，治疗21天时肿瘤大小显著减小，此后7周的所有分析点均如此（p<0.01）。因此，我们认为蜘蛛毒液的体内和体外作用是可以评估的。
