Whiting P H, Midgley M, Dawes E A
J Gen Microbiol. 1976 Feb;92(2):304-10. doi: 10.1099/00221287-92-2-304.
The pathway of glucose metabolism in Pseudomonas aeruginosa was regulated by the availability of glucose and related compounds. On changing from an ammonium limitation to a glucose limitation, the organism responded by adjusting its metabolism substantially from the extracellular direct oxidative pathway to the intracellular phosphorylative route. This change was achieved by repression of the transport systems for gluconate and 2-oxogluconate and of the associated enzymes for 2-oxogluconate metabolism and gluconate kinase, while increasing the levels of glucose transport, hexokinase and glucose 6-phosphate dehydrogenase. The role of gluconate, produced by the action of glucose dehydrogenase, as a major inhibitory factor for glucose transport, and the possible significance of these regulatory mechanisms to the organism in its natural environment, are discussed.
铜绿假单胞菌中葡萄糖代谢途径受葡萄糖及相关化合物可用性的调节。当从铵限制转变为葡萄糖限制时，该生物体通过将其代谢从细胞外直接氧化途径大幅调整为细胞内磷酸化途径做出反应。这种变化是通过抑制葡萄糖酸盐和2-氧代葡萄糖酸盐的转运系统以及2-氧代葡萄糖酸盐代谢和葡萄糖酸激酶的相关酶来实现的，同时提高葡萄糖转运、己糖激酶和葡萄糖6-磷酸脱氢酶的水平。讨论了由葡萄糖脱氢酶作用产生的葡萄糖酸盐作为葡萄糖转运主要抑制因子的作用，以及这些调节机制在自然环境中对该生物体的可能意义。
