Prestes Rosilene Aparecida, Colnago Luiz Alberto, Forato Lucimara Aparecida, Vizzotto Lucinéia, Novotny Etelvino Henrique, Carrilho Emanuel
Universidade de São Paulo, Instituto de Química de São Carlos, Av. Trabalhador São-Carlense 400, São Carlos, SP 13560-970, Brazil.
Anal Chim Acta. 2007 Jul 23;596(2):325-9. doi: 10.1016/j.aca.2007.06.022. Epub 2007 Jun 16.
Oilseeds with modified fatty acid profiles have been the genetic alternative for high quality vegetable oil for food and biodiesel applications. They can provide stable, functional oils for the food industry, without the hydrogenation process that produces trans-fatty acid, which has been linked to cardiovascular disease. High yield and high quality oilseeds are also necessary for the success of biodiesel programs, as polyunsaturated or saturated fatty acid oil produces biofuel with undesirable properties. In this paper, a rapid and automated low resolution NMR method to select intact oilseeds with a modified fatty acid profile is introduced, based on 1H transverse relaxation time (T2). The T2 weighted NMR signal, obtained by a CPMG pulse sequence and processed by chemometric methods was able to determine the oil quality in intact seeds by its fatty composition, cetane number, iodine value and kinematic viscosity with a correlation coefficient r > 0.9. The automated system has the potential to analyze more than 1000 samples per hour and is a powerful tool to speed up the selection of high quality oilseeds for food and biodiesel applications.
具有改良脂肪酸谱的油籽已成为用于食品和生物柴油应用的高品质植物油的遗传替代物。它们可为食品工业提供稳定的功能性油脂，无需氢化过程，而氢化过程会产生与心血管疾病相关的反式脂肪酸。高产和高品质的油籽对于生物柴油项目的成功也很必要，因为多不饱和或饱和脂肪酸油生产的生物燃料具有不良特性。本文介绍了一种基于1H横向弛豫时间（T2）的快速自动化低分辨率核磁共振方法，用于筛选具有改良脂肪酸谱的完整油籽。通过CPMG脉冲序列获得并经化学计量学方法处理的T2加权核磁共振信号，能够根据其脂肪组成、十六烷值、碘值和运动粘度来确定完整种子中的油质，相关系数r>0.9。该自动化系统每小时有潜力分析超过1000个样品，是加速筛选用于食品和生物柴油应用的高品质油籽的有力工具。
