Yeung David K W, Lam Sik L, Griffith James F, Chan Amy B W, Chen Zhenyu, Tsang Polly H, Leung Ping C
Department of Diagnostic Radiology and Organ Imaging, Chinese University of Hong Kong, Prince of Wales Hospital, 30-32 Ngan Shing St, Shatin, Hong Kong (SAR), China.
Chem Phys Lipids. 2008 Feb;151(2):103-9. doi: 10.1016/j.chemphyslip.2007.10.006. Epub 2007 Nov 13.
High-resolution 1H NMR spectroscopy as a complementary method in the analysis of human bone marrow fatty acid (FA) composition was examined. Marrow FA composition in 10 bone samples measured by NMR and gas chromatography (GC) were compared. NMR T1 relaxation time of FA was determined and reproducibility tests were performed to assess the variability. Good correlations were obtained between the NMR and GC results for omega-6 polyunsaturated fatty acid (PUFA) (Spearman r, 0.878), omega-3 PUFA (0.895), monounsaturated FA (0.964) and saturated FA (0.939). The NMR method tended to overestimate saturated FA and underestimate omega-3/omega-6 ratio compared to GC results. T1 relaxation time of marrow FA was 0.56-3.65s. Coefficient of variation of the NMR method was 0.6-8.2% in intra-experimental and 0.2-8.4% in inter-experimental measurements. This study demonstrates a complementary role for 1H NMR spectroscopy as an additional analytical tool in human lipid research.
研究了高分辨率1H核磁共振波谱法作为分析人体骨髓脂肪酸（FA）组成的一种补充方法。比较了通过核磁共振和气相色谱（GC）测量的10个骨样本中的骨髓FA组成。测定了FA的核磁共振T1弛豫时间，并进行了重复性测试以评估变异性。在ω-6多不饱和脂肪酸（PUFA）（斯皮尔曼r，0.878）、ω-3 PUFA（0.895）、单不饱和脂肪酸（0.964）和饱和脂肪酸（0.939）的核磁共振和气相色谱结果之间获得了良好的相关性。与气相色谱结果相比，核磁共振方法倾向于高估饱和脂肪酸并低估ω-3/ω-6比率。骨髓FA的T1弛豫时间为0.56 - 3.65秒。核磁共振方法在实验内测量中的变异系数为0.6 - 8.2%，在实验间测量中的变异系数为0.2 - 8.4%。本研究证明了1H核磁共振波谱法作为人体脂质研究中一种额外分析工具的补充作用。
