乳酸和病原菌在高压处理恢复过程中的蛋白质合成

Protein synthesis in lactic acid and pathogenic bacteria during recovery from a high pressure treatment.

作者信息

Jofré Anna, Champomier-Vergès Marie, Anglade Patricia, Baraige Fabienne, Martín Belén, Garriga Margarita, Zagorec Monique, Aymerich Teresa

机构信息

IRTA, Food Technology, Finca Camps i Armet, E-17121 Monells, Spain.

出版信息

Res Microbiol. 2007 Jul-Aug;158(6):512-20. doi: 10.1016/j.resmic.2007.05.005. Epub 2007 Jun 3.

DOI:10.1016/j.resmic.2007.05.005

PMID:17631981

Abstract

Recovery of injured bacteria after high hydrostatic pressure (HHP) treatment is a key point in food safety. In this study, protein synthesis during the recovery of meat environment bacteria Listeria monocytogenes CTC1011, Lactobacillus sakei 23K, L. sakei CTC494, Enterococcus faecalis CTC6365 and Enterococcus faecium CTC6375 after a 400 MPa HHP treatment was analyzed by two-dimensional gel electrophoresis and peptide mass fingerprinting. After 2 h recovery from HHP treatment, the four species induced transcription factors and proteins related to protein synthesis or fate and enzymes from energy metabolism. However, several stress proteins were specifically induced in the two L. sakei strains. Proteins from the general metabolism predominated in E. faecalis and E. faecium, and stress proteins and proteases predominated in L. monocytogenes. Thus, each species induced a different number of proteins and displayed a specific response which may reflect its specific fitness status.

摘要

高静水压（HHP）处理后受伤细菌的恢复是食品安全中的一个关键点。在本研究中，通过二维凝胶电泳和肽质量指纹图谱分析了400兆帕HHP处理后肉类环境细菌单核细胞增生李斯特菌CTC1011、清酒乳杆菌23K、清酒乳杆菌CTC494、粪肠球菌CTC6365和屎肠球菌CTC6375恢复过程中的蛋白质合成情况。在从HHP处理中恢复2小时后，这四个菌种诱导了与蛋白质合成或命运相关的转录因子和蛋白质以及能量代谢中的酶。然而，在两株清酒乳杆菌中特异性诱导了几种应激蛋白。粪肠球菌和屎肠球菌中一般代谢的蛋白质占主导，而单核细胞增生李斯特菌中应激蛋白和蛋白酶占主导。因此，每个菌种诱导的蛋白质数量不同，并表现出特定的反应，这可能反映了其特定的适应性状态。