具有与其他连接组蛋白不同特性的H1.X是有丝分裂进程所必需的。
H1.X with different properties from other linker histones is required for mitotic progression.
作者信息
Takata Hideaki, Matsunaga Sachihiro, Morimoto Akihiro, Ono-Maniwa Rika, Uchiyama Susumu, Fukui Kiichi
机构信息
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita 565-0871, Japan.
出版信息
FEBS Lett. 2007 Aug 7;581(20):3783-8. doi: 10.1016/j.febslet.2007.06.076. Epub 2007 Jul 10.
We report here the characterization of H1.X, a human histone H1 subtype. We demonstrate that H1.X accumulates in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. In addition, the results of fluorescence recovery after photobleaching indicate that the exchange of H1.X on and off chromatin is faster than that of the other H1 subtypes. Furthermore, RNA interference experiments reveal that H1.X is required for chromosome alignment and segregation. Our results suggest that H1.X has important functions in mitotic progression, which are different from those of the other H1 subtypes.
我们在此报告人类组蛋白H1亚型H1.X的特性。我们证明，H1.X在间期积聚于核仁中，在有丝分裂期间分布于染色体周边。此外，光漂白后荧光恢复的结果表明，H1.X在染色质上的交换进出速度比其他H1亚型更快。此外，RNA干扰实验表明，染色体排列和分离需要H1.X。我们的结果表明，H1.X在有丝分裂进程中具有重要功能，这与其他H1亚型不同。
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