[结核分枝杆菌的分子流行病学——多重耐药菌株与敏感菌株的比较]

[Molecular epidemiology of Mycobacterium tuberculosis--comparison between multidrug-resistant strains and susceptible strains].

作者信息

Yoshida Shiomi, Suzuki Katsuhiro, Tsuyuguchi Kazunari, Okada Masaji, Sakatani Mitsunori

机构信息

Clinical Research Center, National Hospital Organization Kinki-chuo Chest Medical Center, 1180 Nagasone-cho, Kita-ku, Sakai-shi, Osaka 591-8555, Japan.

出版信息

Kekkaku. 2007 Jun;82(6):531-8.

PMID:17633121

Abstract

PURPOSE

Comparing multidrug-resistant M. tuberculosis strains with susceptible strains by molecular epidemiological methods.

METHODS

We examined 109 multidrug-resistant strains (MDR-TB) and 226 susceptible strains (S-TB) derived from National Hospital Organization Kinki-chuo Chest Medical Center by restriction fragment length polymorphism (RFLP) with IS6110, and Spacer oligonucleotide typing (Spoligotyping).

RESULTS

In the case of MDR-TB, 47 strains (43.1%) belonged to 12 descriptions of clusters and the number of IS 6110 copies per isolate ranged from 9 to 25. Similarly, 99 strains (43.8%) belonged to 20 descriptions of clusters in S-TB and the distribution of IS 6110 copies were from 1 to 20. On the other hand, 84 strains of MDR-TB (77.1%) and 191 strains of S-TB (84.5%) belonged to Beijing family by Spoligotyping.

CONCLUSION

MDR and susceptible M. tuberculosis strains were characterized similarities in ratio of clusters by RFLP patterns and high proportion of Beijing family by Spoligotyping. These finding supported the possibility that infectiousness of MDR-TB might be similar to that of S-TB.

摘要

目的

采用分子流行病学方法比较耐多药结核分枝杆菌菌株与敏感菌株。

方法

我们通过IS6110限制性片段长度多态性（RFLP）和间隔寡核苷酸分型（Spoligotyping），检测了来自国立医院组织近畿中央胸部医疗中心的109株耐多药菌株（MDR-TB）和226株敏感菌株（S-TB）。

结果

在耐多药结核病的情况下，47株（43.1%）属于12个聚类描述，每个分离株的IS 6110拷贝数范围为9至25。同样，99株（43.8%）敏感结核病属于20个聚类描述，IS 6110拷贝数分布为1至20。另一方面，通过Spoligotyping分型，84株耐多药结核病（77.1%）和191株敏感结核病（84.5%）属于北京家族。