[Identification of 1,2,4-trichlorobenzene-mineralizing bacteria and their function analysis].

Two strains, E3 and F2, capable to mineralize 1,2,4-trichlorobenzene (1,2,4-TCB) were isolated from a chlorinated benzenes contaminated soil using (14)C-1, 2,4-TCB as carbon source. They were identified by their 16S rDNA coding genes and fluoresence in situ hybridization (FISH) analysis as members of the genus Bordetella. A similarity of 100% were observed between strains E3 and F2 with their 16S rDNA sequences. They had the highest homology of 100% with Bordetella sp. QJ2-5 and the closest relation to described species, Bordetella petrii (GDH030510) with a similary of 99.4%. Strains E3 and F2 could degrade about 90% of 1,2,4-TCB and mineralize 58% and 46% of 1,2,4-TCB to CO2 within 30 days in mineral liquid cultures, respectively. Biomass was formed during the mineralization process.