[小鼠变应性鼻炎合并急性细菌性鼻窦炎的初步研究]

[Preliminary investigation into the allergic rhinitis complicated with acute bacterial sinusitis in mice].

作者信息

An Yun-Fang, Wang Wei-Hua, Zhao Chang-Qing, Xue Jin-Mei, Zhao Hai-Liang

机构信息

Department of Otorhinolaryngology, Second Hospital of Shanxi Medical University, Taiyuan 030001, China.

出版信息

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2007 Feb;42(2):138-42.

PMID:17633259

Abstract

OBJECTIVE

To develop a mouse model of bacterial rhinosinusitis superposed on allergic rhinitis (AR), and to explore whether ongoing allergic rhinitis enhance the acute sinus infection and inflammation associated with Streptococcus pneumoniae (SP).

METHODS

Fourty mice of C57BL6/J were randomly divided on average into 4 groups: A [ovalbumin (OVA) + SP], B [OVA + normal saline (NS)], C [phosphate buffered solution (PBS) + SP] and D (PBS + NS). (1) Group A and B were sensitized by intraperitoneal injection with 200 microl (10%) OVA on days 1 through 9, and exposed to OVA (6%) intranasally on days 10 through 17, to induce allergic inflammation. OVA was replaced with PBS in group C and D in the same way. (2) Subsequently, group A and C were inoculated with SP intranasally on day 13, and NS was used in group B and D. On the 6th day after inoculation, mice were killed. Blood was collected from the orbital venous sinus after anesthesia. The heads were embedded with paraffin and serial sections were followed and stained with hematoxylin-eosin and toluidine blue (0.5%) for histological analysis and inflammation cells count. The number of polymorphonuclear neutrophils (PMN) and eosinophils (EOS) per square millimeter of sinus mucosa were calculated by using a computer-aided special software under microscope.

RESULTS

AR models were successfully established in 9 mice from group A and 8 from group B. Histologic examination of the sinus from group A and B revealed significant mucosal edema and dilated venules. The symptoms were mild in group C, and no symptom was observed in group D. PMN (x +/- s) in group A (139.3 +/- 26.5)/mm2 was significantly higher than that in group B (70.7 +/- 16.7)/mm2, C (63.0 +/- 14.7)/mm2 and D (40.2 +/- 14.1)/mm2 respectively (P < 0.01); EOS and serous IL-5 level in group A (134.6 +/- 25.5)/mm2, (48.2 +/- 13.9) pg/ml and B (116.2 +/- 25.2)/mm2, (40.8 +/- 7.8) pg/ml, were higher than that in group C (16.7 +/- 2.7)/mm2, (23.9 +/- 8.7) pg/ml (P < 0.05) and D (13.4 +/- 4.9)/mm2, (24.6 +/- 6.5) pg/ml (P < 0.05).

CONCLUSIONS

The data demonstrate that an ongoing local allergic response augments bacterial infection in mice, and allergic sensitization alone without SP does not induce the sinus infection.

摘要

目的

建立变应性鼻炎（AR）叠加细菌性鼻窦炎的小鼠模型，探讨持续性变应性鼻炎是否会加重肺炎链球菌（SP）相关的急性鼻窦感染及炎症。

方法

将40只C57BL6/J小鼠平均随机分为4组：A组[卵清蛋白（OVA）+SP]、B组[OVA+生理盐水（NS）]、C组[磷酸盐缓冲液（PBS）+SP]和D组（PBS+NS）。（1）A组和B组于第1至9天腹腔注射200微升（10%）OVA致敏，并于第10至17天经鼻暴露于OVA（6%）以诱导变应性炎症。C组和D组以同样方式用PBS替代OVA。（2）随后，A组和C组于第13天经鼻接种SP，B组和D组用NS。接种后第6天处死小鼠。麻醉后从眶静脉窦取血。将头部用石蜡包埋，制作连续切片，进行苏木精-伊红染色和甲苯胺蓝（0.5%）染色以进行组织学分析和炎症细胞计数。在显微镜下使用计算机辅助专用软件计算每平方毫米鼻窦黏膜中的多形核中性粒细胞（PMN）和嗜酸性粒细胞（EOS）数量。

结果

A组9只小鼠和B组8只小鼠成功建立AR模型。A组和B组鼻窦组织学检查显示黏膜明显水肿和小静脉扩张。C组症状轻微，D组未观察到症状。A组PMN（x±s）为（139.3±26.5）/mm²，显著高于B组（70.7±16.7）/mm²、C组（63.0±14.7）/mm²和D组（40.2±14.1）/mm²（P<0.01）；A组EOS和血清IL-5水平分别为（134.6±25.5）/mm²、（48.2±13.9）pg/ml，B组为（116.2±25.2）/mm²、（40.8±7.8）pg/ml，高于C组（16.7±2.7）/mm²、（23.9±8.7）pg/ml（P<0.05）和D组（13.4±4.9）/mm²、（24.6±6.5）pg/ml（P<0.05）。