Luxameechanporn Thongchai, Blair Christopher, Kirtsreesakul Virat, Thompson Kenneth, Naclerio Robert M
Section of Otolaryngology-Head and Neck Surgery, University of Chicago, 5841 South Maryland Avenue, MC 1035, Chicago, IL 60637, USA.
Int J Infect Dis. 2006 Sep;10(5):401-6. doi: 10.1016/j.ijid.2005.07.007. Epub 2006 Mar 24.
Acute bacterial rhinosinusitis, which is a major health problem, is treated with antibiotics. We developed a mouse model of acute bacterial rhinosinusitis to gain a better understanding of the pathophysiology of the disease. Our goal was to investigate the response to acute rhinosinusitis when treated with either a bactericidal or a bacteriostatic antibiotic.
C57BL/6 mice were infected intranasally with Streptococcus pneumoniae. One day after inoculation, the mice were treated with either moxifloxacin (bactericidal) or azithromycin (bacteriostatic). Different groups were euthanized during the first five days post-inoculation. Bacterial counts from nasal lavage culture and the cell markers GR1, CD11b, CD3, CD4, and CD8 in sinus tissue were evaluated by flow cytometry.
Azithromycin led to rapid clearance of the bacteria and of the inflammation in contrast to placebo. Surprisingly, moxifloxacin showed a limited effect. Investigations of this limited effect of moxifloxacin suggested a high metabolic clearance, a low concentration at the site of infection, and low persistent post-antibiotic effects of moxifloxacin in mice.
Our animal model of acute sinusitis has great utility for studying the disease, but the difference between mice and man must always be considered in making extrapolations from animal experiments to the human experience.
急性细菌性鼻窦炎是一个主要的健康问题，通常使用抗生素进行治疗。我们建立了一种急性细菌性鼻窦炎小鼠模型，以更好地了解该疾病的病理生理学。我们的目标是研究用杀菌性或抑菌性抗生素治疗急性鼻窦炎时的反应。
将肺炎链球菌经鼻内感染C57BL/6小鼠。接种后一天，小鼠分别用莫西沙星（杀菌性）或阿奇霉素（抑菌性）进行治疗。在接种后的前五天内，对不同组的小鼠实施安乐死。通过流式细胞术评估鼻腔灌洗培养物中的细菌计数以及鼻窦组织中的细胞标志物GR1、CD11b、CD3、CD4和CD8。
与安慰剂相比，阿奇霉素能迅速清除细菌和炎症。令人惊讶的是，莫西沙星的效果有限。对莫西沙星这种有限效果的研究表明，其在小鼠体内具有高代谢清除率、感染部位浓度低以及抗生素后效应持续时间短的特点。
我们的急性鼻窦炎动物模型在研究该疾病方面具有很大的实用性，但在从动物实验推断人类情况时，必须始终考虑小鼠与人类之间的差异。
