Yang Ke-hong, Ge Shu-xing, Xu Bing-ying, Yan Jun-ling, Wu Lan-ou
Department of Pharmacology, Kunming Medical College, Kunming 650031, China.
Zhong Yao Cai. 2007 Mar;30(3):313-6.
To study the effect of notoginsenoside-Rg, on expression of BDNF mRNA (brain-derived neurotrophic factor messenger ribonucleic acid) in cerebrum cortex after MCAO/R (middle cerebral artery occlusion reperfusion) injury in rat by real-time quantitative polymerase chain reaction.
36 SD male rats were randomly divided into MCAO/R model group, notogisenoside-Rg1 therapy group (100 mg/kg) and the positive medicine control group (nimodipine 1 mg/kg). The MCAO/R model were made by thread-occluded method. The four rats were randomly taken from each groups and were killed to be breaken out brain tissues as specimens after which were treated with medicine in 1,3, 5 days. Total RNA was isolated from cerebrum cortex. Specific oligonucleotide primers of BDNF mRNA gene fragments were amplificated by RT-PCR to construct recombinant plasmid and sequence. To dilute recombinant plasmid didploidly and a quantitative standard curve was completed. Taqman fluorogenic quantitative RT-PCR (FQ-PCR) was set up to detect the BDNF mRNA variety of cerebrum cortex.
Compared with the model group and the postive control group, notogisenoside-Rg1 treated groups could obviously improve some nervous deficit symptoms in the cerebral ischemia and increase BDNF mRNA amount that in the cerebrum cortex at different period after rat MCAO/R injury.
Notoginsenoside-Rg1 can promote to generating internal BDNF protein in brain by up-regulation the expression of BDNF mRNA amount in the cerebrum cortex. BDNF bind in TrkB, which can give rise to protective effects for ischemic neurons by generating corresponding domino effect molecules. Accordingly it can be as a therapy method in cerebral ischemia injury.
采用实时定量聚合酶链反应研究三七皂苷Rg1对大鼠大脑中动脉闭塞/再灌注（MCAO/R）损伤后大脑皮质脑源性神经营养因子信使核糖核酸（BDNF mRNA）表达的影响。
将36只雄性SD大鼠随机分为MCAO/R模型组、三七皂苷Rg1治疗组（100mg/kg）和阳性药物对照组（尼莫地平1mg/kg）。采用线栓法制备MCAO/R模型。每组随机取4只大鼠，于给药1、3、5天后处死并取脑组织标本。从大脑皮质提取总RNA。通过逆转录聚合酶链反应（RT-PCR）扩增BDNF mRNA基因片段的特异性寡核苷酸引物，构建重组质粒并测序。将重组质粒进行双倍稀释，完成定量标准曲线。建立Taqman荧光定量逆转录聚合酶链反应（FQ-PCR）检测大脑皮质BDNF mRNA的变化。
与模型组和阳性对照组相比，三七皂苷Rg1治疗组能明显改善大鼠MCAO/R损伤后不同时期的脑缺血神经功能缺损症状，并增加大脑皮质BDNF mRNA含量。
三七皂苷Rg1可通过上调大脑皮质BDNF mRNA表达，促进脑内BDNF蛋白的生成。BDNF与酪氨酸激酶受体B（TrkB）结合，通过产生相应的多米诺效应分子对缺血神经元产生保护作用。因此，可作为脑缺血损伤的一种治疗方法。
