Miyoshi Goichi, Butt Simon J B, Takebayashi Hirohide, Fishell Gord
Neuroscience Program and Department of Cell Biology, Smilow Research Center, New York University School of Medicine, New York, New York 10016, USA.
J Neurosci. 2007 Jul 18;27(29):7786-98. doi: 10.1523/JNEUROSCI.1807-07.2007.
Inhibitory GABAergic interneurons of the mouse neocortex are a highly heterogeneous population of neurons that originate from the ventral telencephalon and migrate tangentially up into the developing cortical plate. The majority of cortical interneurons arise from a transient embryonic structure known as the medial ganglionic eminence (MGE), but how the remarkable diversity is specified in this region is not known. We have taken a genetic fate mapping strategy to elucidate the temporal origins of cortical interneuron subtypes within the MGE. We used an inducible form of Cre under the regulation of Olig2, a basic helix-loop-helix transcription factor highly expressed in neural progenitors of the MGE. We observe that the physiological subtypes of cortical interneurons are, to a large degree, unique to their time point of generation.
小鼠新皮层中的抑制性γ-氨基丁酸能中间神经元是一类高度异质性的神经元群体，它们起源于腹侧端脑，并沿切线方向向上迁移至发育中的皮质板。大多数皮质中间神经元起源于一个称为内侧神经节隆起（MGE）的短暂胚胎结构，但该区域如何产生如此显著的多样性尚不清楚。我们采用了一种基因命运图谱策略来阐明MGE内皮质中间神经元亚型的时间起源。我们使用了一种在Olig2调控下的可诱导型Cre，Olig2是一种在MGE的神经祖细胞中高度表达的碱性螺旋-环-螺旋转录因子。我们观察到，皮质中间神经元的生理亚型在很大程度上对于它们的产生时间点是独特的。
