基于小麦胚无细胞表达系统的高通量遗传信息实现方法。

Methods for high-throughput materialization of genetic information based on wheat germ cell-free expression system.

作者信息

Sawasaki Tatsuya, Morishita Ryo, Gouda Mudeppa D, Endo Yaeta

机构信息

Cell-Free Science and Technology Research Center, Ehime University, Matsuyama, Japan.

出版信息

Methods Mol Biol. 2007;375:95-106. doi: 10.1007/978-1-59745-388-2_5.

DOI:10.1007/978-1-59745-388-2_5

PMID:17634598

Abstract

Among the cell-free protein synthesis systems, the wheat germ-based translation system has significant advantages for the high-throughput production of eukaryotic multidomain proteins in folded state. Here, we describe protocols for this cell-free expression system.

摘要

在无细胞蛋白质合成系统中，基于小麦胚芽的翻译系统在高通量生产折叠状态的真核多结构域蛋白质方面具有显著优势。在此，我们描述了该无细胞表达系统的实验方案。