监测G蛋白偶联受体与β-抑制蛋白之间的相互作用。

Monitoring interactions between G-protein-coupled receptors and beta-arrestins.

作者信息

Pfleger K D G, Dalrymple M B, Dromey J R, Eidne K A

机构信息

7TM Laboratory/Laboratory for Molecular Endocrinology, Western Australian Institute for Medical Research (WAIMR) and Centre for Medical Research, University of Western Australia, Nedlands, Perth, WA 6009, Australia.

出版信息

Biochem Soc Trans. 2007 Aug;35(Pt 4):764-6. doi: 10.1042/BST0350764.

DOI:10.1042/BST0350764

PMID:17635143

Abstract

beta-Arrestins 1 and 2 are ubiquitously expressed intracellular adaptor and scaffolding proteins that play important roles in GPCR (G-protein-coupled receptor) desensitization, internalization, intracellular trafficking and G-protein-independent signalling. Recent developments in BRET (bioluminescence resonance energy transfer) technology enable novel insights to be gained from real-time monitoring of GPCR-beta-arrestin complexes in live cells for prolonged periods. In concert with confocal microscopy, assays for studying internalization and recycling kinetics such as ELISAs, and techniques for measuring downstream signalling pathways such as those involving MAPKs (mitogen-activated protein kinases), investigators can now use a range of experimental tools to elucidate the ever-expanding roles of beta-arrestins in mediating GPCR function.

摘要

β抑制蛋白1和2是广泛表达的细胞内衔接蛋白和支架蛋白，在G蛋白偶联受体（GPCR）脱敏、内化、细胞内转运以及非G蛋白依赖性信号传导中发挥重要作用。生物发光共振能量转移（BRET）技术的最新进展使得能够通过长时间实时监测活细胞中的GPCR-β抑制蛋白复合物获得新的见解。结合共聚焦显微镜、用于研究内化和循环动力学的检测方法（如酶联免疫吸附测定法）以及用于测量下游信号通路的技术（如涉及丝裂原活化蛋白激酶的技术），研究人员现在可以使用一系列实验工具来阐明β抑制蛋白在介导GPCR功能方面不断扩展的作用。