Scarlett Christopher J, Samra Jaswinder S, Xue Aiqun, Baxter Robert C, Smith Ross C
Department of Surgery, University of Sydney, Sydney, New South Wales, Australia.
ANZ J Surg. 2007 Aug;77(8):648-53. doi: 10.1111/j.1445-2197.2007.04179.x.
The diagnosis of pancreatic cystic lesions is problematical with difficulties arising in the differentiation between malignant, premalignant or benign lesions. This preliminary study aimed to analyse pancreatic cyst fluid, using a proteomic approach, to generate reproducible protein profiles to assist in the classification of malignant and non-carcinoma samples.
Pancreatic cyst fluid samples from patients with pancreatic adenocarcinoma and non-carcinoma cystic lesions were analysed on hydrophobic protein chip arrays by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS).
Differential protein expression profiles were observed between pancreatic adenocarcinoma and non-carcinoma cyst fluid samples using SELDI-TOF MS, with 12 protein peaks differentially expressed between pancreatic adenocarcinoma and non-carcinoma. Additionally, unique patterns were observed between the different subtypes of non-carcinoma samples as well as malignant adenocarcinoma.
In this preliminary study we used SELDI-TOF MS to identify protein expression profiles of pancreatic cyst fluid, showing a potential to aid in the differential diagnosis of pancreatic cystic lesions.
胰腺囊性病变的诊断存在问题，难以区分恶性、癌前或良性病变。这项初步研究旨在采用蛋白质组学方法分析胰腺囊肿液，以生成可重复的蛋白质谱，辅助对恶性和非癌样本进行分类。
通过表面增强激光解吸/电离飞行时间质谱（SELDI-TOF MS），在疏水蛋白质芯片阵列上分析胰腺腺癌患者和非癌性囊性病变患者的胰腺囊肿液样本。
使用SELDI-TOF MS观察到胰腺腺癌和非癌性囊肿液样本之间存在差异蛋白质表达谱，胰腺腺癌和非癌之间有12个蛋白质峰差异表达。此外，在非癌样本的不同亚型以及恶性腺癌之间也观察到独特的模式。
在这项初步研究中，我们使用SELDI-TOF MS鉴定胰腺囊肿液的蛋白质表达谱，显示出有助于胰腺囊性病变鉴别诊断的潜力。
