Păunescu Virgil, Deak Erika, Herman Diana, Siska Ioana Raluca, Tănasie Gabriela, Bunu Carmen, Anghel Simona, Tatu Calin A, Oprea Tudor I, Henschler Reinhard, Rüster Brigitte, Bistrian Roxana, Seifried Erhard
Department of Physiology and Immunology, University of Medicine and Pharmacy Victor Babes Timisoara, Romania.
J Cell Mol Med. 2007 May-Jun;11(3):502-8. doi: 10.1111/j.1582-4934.2007.00041.x.
Our study examined whether human bone marrow-derived MSCs are able to differentiate, in vitro, into functional epithelial-like cells. MSCs were isolated from the sternum of 8 patients with different hematological disorders. The surface phenotype of these cells was characterized.To induce epithelial differentiation, MSCs were cultured using Epidermal Growth Factor, Keratinocyte Growth Factor, Hepatocyte Growth Factor and Insulin-like growth Factor-II. Differentiated cells were further characterized both morphologically and functionally by their capacity to express markers with specificity for epithelial lineage. The expression of cytokeratin 19 was assessed by immunocytochemistry, and cytokeratin 18 was evaluated by quantitative RT-PCR (Taq-man). The data demonstrate that human MSCs isolated from human bone marrow can differentiate into epithelial-like cells and may thus serve as a cell source for tissue engineering and cell therapy of epithelial tissue.
我们的研究检测了人骨髓间充质干细胞在体外是否能够分化为功能性上皮样细胞。从8例患有不同血液系统疾病的患者胸骨中分离出间充质干细胞。对这些细胞的表面表型进行了鉴定。为诱导上皮分化，使用表皮生长因子、角质形成细胞生长因子、肝细胞生长因子和胰岛素样生长因子-II培养间充质干细胞。通过分化细胞表达上皮谱系特异性标志物的能力，从形态学和功能上对其进行进一步鉴定。通过免疫细胞化学评估细胞角蛋白19的表达，并通过定量逆转录聚合酶链反应（Taq-man）评估细胞角蛋白18。数据表明，从人骨髓分离出的人间充质干细胞可分化为上皮样细胞，因此可作为上皮组织工程和细胞治疗的细胞来源。
