Leroux O, Van der Kinderen G, Viane R L L
Department of Biology, Research group Pteridology, Ghent University, K.L., Ledeganckstraat 35, B-9000 Gent, Belgium.
J Microsc. 2007 Jul;227(Pt 1):79-82. doi: 10.1111/j.1365-2818.2007.01786.x.
High-quality sections are indispensable for many scientific studies. Most published methods are often time-consuming or require special devices. We present an easy, quick and low-cost method for oriented embedding of thin structures using glycol methacrylate resin and self-constructed, reusable embedding tools made of overhead transparencies. This technique allows for more flexibility in orientation than other methods, enabling precise transverse, longitudinal and even oblique sectioning.
高质量切片对于许多科学研究来说不可或缺。大多数已发表的方法往往耗时较长或需要特殊设备。我们提出了一种简便、快速且低成本的方法,用于使用甲基丙烯酸乙二醇酯树脂和由透明投影片自制的可重复使用的包埋工具对薄结构进行定向包埋。与其他方法相比,该技术在方向定位上具有更大的灵活性,能够实现精确的横向、纵向甚至斜向切片。