Furie B, Furie B C
Division of Hemostasis and Thrombosis, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02215, USA.
J Thromb Haemost. 2007 Jul;5 Suppl 1:12-7. doi: 10.1111/j.1538-7836.2007.02482.x.
Thrombus formation, including platelet adhesion, activation, secretion and aggregation as well as tissue factor-initiated thrombin generation and fibrin formation, has been studied in the past using in vitro systems, often with isolated components. Given the complexity of hemostasis and thrombosis, many of the concepts that have been developed to explain these processes are being revisited by studying thrombus formation in live animals using intravital microscopy and genetically altered mice. Although much of the dogma that has evolved has been confirmed by in vivo studies of thrombus formation, there have also been conflicts between old concepts and new direct observations. In vivo studies of the initiation of thrombus formation, platelet accumulation and thrombin generation have provided evidence for the participation of novel proteins and identified new pathways and mechanisms.
过去曾使用体外系统，通常是分离的成分，来研究血栓形成，包括血小板黏附、活化、分泌和聚集，以及组织因子启动的凝血酶生成和纤维蛋白形成。鉴于止血和血栓形成的复杂性，许多为解释这些过程而提出的概念，正通过活体显微镜和基因改造小鼠，在活体动物中研究血栓形成来重新审视。尽管已发展的许多教条已通过血栓形成的体内研究得到证实，但旧概念与新的直接观察结果之间也存在冲突。血栓形成起始、血小板聚集和凝血酶生成的体内研究，为新蛋白质的参与提供了证据，并确定了新的途径和机制。
