Egerszegi I, Hazeleger W, Rátky J, Sarlós P, Kemp B, Bouwman E, Solti L, Brüssow K-P
Research Institute for Animal Breeding and Nutrition, Herceghalom, Hungary.
Reprod Domest Anim. 2007 Aug;42(4):441-4. doi: 10.1111/j.1439-0531.2006.00790.x.
The aim of the study was to compare how different feeding levels affect the ovarian potential of follicular development and oocyte maturation in response to superovulatory treatment in native Mangalica (M, n = 17) compared with Landrace (L, n = 20) pigs. Gilts of both breeds were fed high-energy (HI-2.5 kg) or low-energy (LO - 1.25 kg) feed during oestrus synchronization (15 days of Regumate feeding) till the time of oocyte aspiration (Day 6 after Regumate). Follicular growth was stimulated by the administration of 1000 IU equiue choriou gonadotropiu (eCG) 24 h after Regumate treatment, and ovulation was induced by injection of 750 IU human choriou gonadotropiu (hCG) 80 h after eCG administration. Ultrasound (US) investigation was done three times (4-10 h before, and 40-44 and 72-74 h after eCG administration) for the observation of follicular development. Oocyte and follicular fluid (FF) were collected endoscopically 34 h after hCG injection. Cumulus-oocyte complexes were evaluated, their morphology determined, and thereafter fixed and stained for chromatin evaluation. Oocytes were classified as meiosis-resumed (germinal vesicle breakdown, diakinesis, metaphase I to anaphase I) or matured (telophase I and metaphase II). FF concentrations of oestradiol and progesterone were measured by validated radioimmunoassays. In L gilts, differences were observed between HI and LO in the number of preovulatory follicles (32.3 +/- 10.5 vs 17.1 +/- 12.3, p < 0.05), but not in M (25.3 +/- 2.9 vs 28.8 +/- 7.3, p > 0.05). Initial follicular growth was not affected by feeding levels; however, preovulatory follicle size was larger in M (7.1 +/- 0.9 and 6.9 +/- 1.1 mm vs 5.7 +/- 0.7 and 5.5 +/- 0.8 mm; p < 0.05). No differences were obtained with relation to mature chromatin configuration in both breeds (L gilts: HI - 70% and LO-67% vs M gilts: HI - 67% and LO - 63%). A twofold higher oestradiol concentration was detected in FF of HI-M and LO-M (29.6 +/- 6.8 and 30.9 +/- 10.3 ng/ml respectively) compared with that of L (16.9 +/- 9.7 and 17.9 +/- 3.6 ng/ml, respectively; p < 0.05). The mean FF progesterone level was nearly fivefold higher in M (2020.4 +/- 1056 and 1512.2 +/- 1121.8 ng/ml) compared with L (386.2 +/- 113.7 and 298.8 +/- 125.9 ng/ml, p < 0.05). The results indicate an influence of the feeding of altered energy on the number of recruitable preovulatory follicles in modern Landrace but not in native Mangalica breed. Moreover, the follicular steroid hormone milieu differs between Landrace and Mangalica gilts but not depending on feeding levels. Oocyte maturation was not affected by diet.
本研究的目的是比较不同饲养水平对本地曼加利察猪（M，n = 17）和长白猪（L，n = 20）在超数排卵处理下卵泡发育的卵巢潜能和卵母细胞成熟的影响。在发情同步化期间（饲喂雷古梅15天）直至卵母细胞抽吸时（雷古梅处理后第6天），两个品种的后备母猪均饲喂高能（HI - 2.5 kg）或低能（LO - 1.25 kg）饲料。在雷古梅处理后24小时给予1000 IU马绒毛膜促性腺激素（eCG）刺激卵泡生长，并在eCG给药后80小时注射750 IU人绒毛膜促性腺激素（hCG）诱导排卵。超声（US）检查进行了三次（eCG给药前4 - 10小时，以及eCG给药后40 - 44小时和72 - 74小时）以观察卵泡发育。在hCG注射后34小时通过内窥镜收集卵母细胞和卵泡液（FF）。评估卵丘 - 卵母细胞复合体，确定其形态，然后固定并染色以进行染色质评估。卵母细胞分为减数分裂恢复型（生发泡破裂、终变期、减数第一次分裂中期至后期）或成熟型（减数第一次分裂末期和减数第二次分裂中期）。通过经过验证的放射免疫测定法测量FF中雌二醇和孕酮的浓度。在L后备母猪中，HI和LO之间在排卵前卵泡数量上存在差异（32.3±10.5对17.1±12.3，p < 0.05），但在M中没有差异（25.3±2.9对28.8±7.3，p > 0.05）。初始卵泡生长不受饲养水平影响；然而，M中排卵前卵泡大小更大（7.1±0.9和6.9±1.1 mm对5.7±0.7和5.5±0.8 mm；p < 0.05）。两个品种在成熟染色质构型方面没有差异（L后备母猪：HI - 70%和LO - 67%对M后备母猪：HI - 67%和LO - 63%）。与L相比，HI - M和LO - M的FF中检测到的雌二醇浓度高出两倍（分别为29.6±6.8和30.9±10.3 ng/ml对16.9±9.7和17.9±3.6 ng/ml；p < 0.05）。M的FF中孕酮平均水平比L高出近五倍（2020.4±1056和1512.2±1121.8 ng/ml对386.2±113.7和298.8±125.9 ng/ml，p < 0.05）。结果表明，改变能量的饲养对现代长白猪可募集的排卵前卵泡数量有影响，但对本地曼加利察品种没有影响。此外，长白猪和曼加利察后备母猪之间的卵泡类固醇激素环境不同，但不受饲养水平影响。饮食对卵母细胞成熟没有影响。
