Yang Zhaohai, Harrison Keith, Park Yara A, Chaffin Carolyn H, Thigpen Beatrice, Easley Pattye L, Smith John A, Robinson C Andrew, Lorenz Robin G, Hardy Robert W
Department of Pathology, University of Alabama, Birmingham, AL 35233, USA.
Am J Clin Pathol. 2007 Aug;128(2):293-9. doi: 10.1309/1L3CG8GK6F8VYNYH.
We evaluated the performance of the CAPILLARYS 2 (Sebia, Norcross, GA) capillary electrophoresis system for detection and identification of monoclonal proteins in serum samples. We analyzed 104 serum specimens by Sebia Hydragel serum protein electrophoresis (agarose gel electrophoresis [AGE]/immunofixation electrophoresis [IFE]) and CAPILLARYS 2 capillary zone electrophoresis (CZE)/immunosubtraction. AGE and CZE had sensitivities of 90% and 81%, respectively, based on IFE as the "gold standard," and all bands detected were confirmed by IFE (100% specificity). AGE and CZE had an overall agreement of 91% on serum protein electrophoresis. In the population tested, IgG was detected in 29% of samples by IFE and 30% using immunosubtraction. Similarly IgA was detected in 9% of cases by IFE and 8% by immunosubtraction. IgM and light chains were detected in 6% and 3% of cases, respectively, by IFE, whereas CZE/immunosubtraction did not detect any IgM or light chains. In our hands, AGE and CZE had the same specificity for detection of monoclonal proteins; however, CZE/immunosubtraction seems to be less sensitive than IFE for the detection of IgM and, possibly, serum light chains.
我们评估了CAPILLARYS 2（Sebia公司，诺克罗斯，佐治亚州）毛细管电泳系统在血清样本中检测和鉴定单克隆蛋白的性能。我们采用Sebia Hydragel血清蛋白电泳（琼脂糖凝胶电泳[AGE]/免疫固定电泳[IFE]）以及CAPILLARYS 2毛细管区带电泳（CZE）/免疫扣除法对104份血清标本进行了分析。以IFE作为“金标准”，AGE和CZE的灵敏度分别为90%和81%，并且所有检测到的条带均通过IFE得到确认（特异性为100%）。在血清蛋白电泳方面，AGE和CZE的总体一致性为91%。在所检测的人群中，通过IFE在29%的样本中检测到IgG，采用免疫扣除法检测到的比例为30%。同样，通过IFE在9%的病例中检测到IgA，免疫扣除法检测到的比例为8%。通过IFE分别在6%和3%的病例中检测到IgM和轻链，而CZE/免疫扣除法未检测到任何IgM或轻链。在我们的研究中，AGE和CZE在检测单克隆蛋白方面具有相同的特异性；然而，CZE/免疫扣除法在检测IgM以及可能的血清轻链方面似乎不如IFE敏感。
