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临床环境中琼脂糖凝胶血清蛋白电泳与毛细管区带电泳的比较。

Comparison of serum protein electrophoresis by agarose gel and capillary zone electrophoresis in a clinical setting.

作者信息

Jolliff C R, Blessum C R

机构信息

Immunology Physician's Laboratory Services, Nebraska Wesleyan University, Lincoln 68500, USA.

出版信息

Electrophoresis. 1997 Sep;18(10):1781-4. doi: 10.1002/elps.1150181012.

Abstract

Capillary zone electrophoresis (CZE) offers the potential for automating serum protein electrophoretic analysis traditionally performed on standard thin-layer agarose gels. The following describes the use of CZE compared to agarose gel electrophoresis (AGE) for the detection of dysproteinemia and paraproteinemia in a clinical study involving 240 patients. The study includes within-run and between-run reproducibility data on the Paragon CZE 2000 Clinical Capillary Electrophoresis System, in addition to concordance data between the two methodologies. Paraprotein quantitation studies comparing AGE versus CZE were also performed. Reproducibility for the automated CZE system was superior to the AGE system. Improved reproducibility for the CZE method is largely due to measuring protein absorbance directly at 214 nm versus the traditional AGE method that measures the amount of dye adsorbed to protein. Reproducibility data as percent coefficient of variance (% CV) for the five classic bands in a normal control serum for between-run precision ranged from 1.2 to 4.5% for CZE compared to AGE, which ranged from 3.8 to 8.0% CV. Concordance studies between AGE and CZE involving dysproteinemias including hypogammaglobulinemia, polyclonal and monoclonal gammopathies, acute and chronic inflammation, nephrosis, hepatodegenerative disease, cirrhosis, and iron deficiency anemia showed 96% agreement. Paraprotein classification, which compared the CZE immunosubtraction method to immunofixation electrophoresis (IFE) on agarose, showed 100% agreement. Certain dysproteinemias involving beta lipoprotein were in partial concordance due to the inability of the CZE procedure to detect this component. Detection limits for monoclonal gammopathies, providing they were not comigrating with other proteins, were IgG 50 mg/dL, IgM 75 mg/dL, and IgA 75 mg/dL. Paraprotein quantitative studies between the two methods showed less than a +/- 0.2 g/dL variation.

摘要

毛细管区带电泳(CZE)为传统上在标准薄层琼脂糖凝胶上进行的血清蛋白电泳分析自动化提供了可能。以下介绍了在一项涉及240名患者的临床研究中,将CZE与琼脂糖凝胶电泳(AGE)用于检测异常蛋白血症和副蛋白血症的情况。该研究包括Paragon CZE 2000临床毛细管电泳系统的批内和批间重现性数据,以及两种方法之间的一致性数据。还进行了比较AGE与CZE的副蛋白定量研究。自动化CZE系统的重现性优于AGE系统。CZE方法重现性的提高很大程度上是因为直接在214nm处测量蛋白质吸光度,而传统的AGE方法是测量吸附到蛋白质上的染料量。正常对照血清中五个经典条带的批间精密度的重现性数据以变异系数百分比(%CV)表示,CZE为1.2%至4.5%,而AGE为3.8%至8.0%CV。AGE和CZE之间涉及异常蛋白血症的一致性研究,包括低丙种球蛋白血症、多克隆和单克隆丙种球蛋白病、急慢性炎症、肾病、肝退行性疾病、肝硬化和缺铁性贫血,显示一致性为96%。将CZE免疫扣除法与琼脂糖上的免疫固定电泳(IFE)进行比较的副蛋白分类显示一致性为100%。某些涉及β脂蛋白的异常蛋白血症存在部分一致性,因为CZE程序无法检测到该成分。单克隆丙种球蛋白病的检测限(前提是它们不与其他蛋白质共迁移)为IgG 50mg/dL、IgM 75mg/dL和IgA 75mg/dL。两种方法之间的副蛋白定量研究显示差异小于±0.2g/dL。

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