Lin Chia Ling, Chiu Cheng Hsun, Chu Chishih, Huang Yhu Chering, Lin Tzou Yien, Ou Jonathan T
Department of Pediatrics, Chang Gung Children's Hospital, Taoyuan, Taiwan.
J Microbiol Immunol Infect. 2007 Jun;40(3):222-6.
Salmonella enterica is one of the most common enteric pathogens worldwide. Conventional methods of isolation of Salmonella strains take 4-7 days to complete, are laborious and require substantial manpower. We devised a polymerase chain reaction (PCR) method that simultaneously uses three pairs of specific primers to detect inv, spv, and via genes of Salmonella.
Three primer pairs were designed, including: SPVC-1 and SPVC-2, based on the nucleotide sequences of the spvC gene; INVA-1 and INVA-2, based on the invA gene; and VIAB-1 and VIAB-2, based on the viaB gene. PCR was performed using these three primers to identify 14 clinically important bacterial organisms, including Citrobacter freundii, S. enterica serovars Typhi and Paratyphi C, Dublin, and other non-typhoidal Salmonella that harbor a virulence plasmid.
The following strains were readily identified using the PCR: (1) C. freundii; (2) S. Typhi; and S. Paratyphi C; (3) S. Dublin (virulence antigen-positive); and (4) Salmonella serovars that harbor an spv-type virulence plasmid. S. enterica could also be identified, but required further testing to determine serovar.
This PCR method allows S. Typhi to be identified immediately so that appropriate antibiotic treatment can be initiated without delay.
肠炎沙门氏菌是全球最常见的肠道病原体之一。传统的沙门氏菌菌株分离方法需要4至7天才能完成，操作繁琐且需要大量人力。我们设计了一种聚合酶链反应（PCR）方法，该方法同时使用三对特异性引物来检测沙门氏菌的inv、spv和via基因。
设计了三对引物，包括：基于spvC基因核苷酸序列的SPVC-1和SPVC-2；基于invA基因的INVA-1和INVA-2；以及基于viaB基因的VIAB-1和VIAB-2。使用这三对引物进行PCR，以鉴定14种临床重要的细菌，包括弗氏柠檬酸杆菌、伤寒沙门氏菌血清型伤寒和副伤寒C、都柏林沙门氏菌以及其他携带毒力质粒的非伤寒沙门氏菌。
使用PCR可轻松鉴定以下菌株：（1）弗氏柠檬酸杆菌；（2）伤寒沙门氏菌；和副伤寒C沙门氏菌；（3）都柏林沙门氏菌（毒力抗原阳性）；以及（4）携带spv型毒力质粒的沙门氏菌血清型。肠炎沙门氏菌也可以被鉴定出来，但需要进一步检测以确定血清型。
这种PCR方法可以立即鉴定出伤寒沙门氏菌，从而能够立即开始适当的抗生素治疗。
