Levy Haim, Diallo Souleymane, Tennant Sharon M, Livio Sofie, Sow Samba O, Tapia Milagritos, Fields Patricia I, Mikoleit Matthew, Tamboura Boubou, Kotloff Karen L, Lagos Rosanna, Nataro James P, Galen James E, Levine Myron M
Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
J Clin Microbiol. 2008 May;46(5):1861-6. doi: 10.1128/JCM.00109-08. Epub 2008 Mar 26.
PCR methodology was developed to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B. One multiplex PCR identifies serogroup D, A, and B and Vi-positive strains; another confirms flagellar antigen "d," "a," or "b." Blinded testing of 664 Malian and Chilean Salmonella blood isolates demonstrated 100% sensitivity and specificity.
聚合酶链反应(PCR)方法被开发用于鉴定伤寒沙门氏菌、甲型副伤寒沙门氏菌和乙型副伤寒沙门氏菌血清型。一种多重PCR可鉴定D群、A群和B群以及Vi阳性菌株;另一种则可确认鞭毛抗原“d”、“a”或“b”。对664份马里和智利沙门氏菌血液分离株进行的盲法检测显示,其灵敏度和特异性均为100%。