Duygulu Fuat, Yakan Birkan, Karaoglu Sinan, Kutlubay Recep, Karahan Okkes I, Ozturk Ahmet
Department of Orthopedics, Erciyes University Medical Faculty, Kayseri, Turkey.
Arch Orthop Trauma Surg. 2007 Sep;127(7):493-501. doi: 10.1007/s00402-007-0395-7. Epub 2007 Jul 17.
To investigate the effects of free oxygen radicals and various antioxidants on bone healing after experimental formation of fracture.
Fifty male rats were used and divided into five groups (ten rats in each). The right forelimbs of the rats were broken by bimanual compression method. One hour before this procedure, 5 ml/kg of intraperitoneal (i.p.) physiologic saline were given to the control Group 1. All 40 rats in the experimental Groups 2, 3, 4 and 5 were treated with i.p. zymosan at a dosage of 100 mg/kg to induce the production of free radicals by stimulating NADPH oxidase in polymorphonuclear leukocytes. Zymosan induction was stopped on the fifth post-fracture day. In addition to the zymosan, i.p. 1 g/kg/day of dimethyl sulfoxide were given to the animals in Group 3, 50 mg/kg/d of Ginko biloba Extract (EGb 761) in Group 4 and 500 mg/kg/day of vitamin C in Group 5. Radiographs of the fractures of all animals were obtained to assess callus formation, remodeling and bridging bone formation under ether anesthetics on postfracture day 7, 14 and 21. All rats were euthanized on day 22, and sections of the radius and ulna were examined both histologically with light and electron microscopy and ultrastructurally. Statistical analysis was made with Kruskal-Wallis variance analyze test and comparison between groups was performed by Dunn's multiple comparison test.
An impairment of bone healing was observed in Group 2 inducted with purely zymosan. Variable results were obtained for bone healing in the groups treated with various antioxidants. There was very significant difference of fracture healing between Groups 1 and 2 both histologically and radiologically (P < 0.001). There was significant difference between Groups 2 and 5 radiologically (P < 0.05).
Free oxygen radicals demonstrate a negative effect on fracture healing and vitamin C (an antioxidant) partially prevents the negative effect of zymosan on fracture healing.
研究游离氧自由基及多种抗氧化剂对实验性骨折后骨愈合的影响。
选用50只雄性大鼠，分为五组（每组10只）。采用双手压迫法使大鼠右前肢骨折。在该操作前1小时，给第1对照组腹腔注射5 ml/kg的生理盐水。第2、3、4和5实验组的40只大鼠均腹腔注射100 mg/kg的酵母聚糖，以通过刺激多形核白细胞中的NADPH氧化酶诱导自由基生成。骨折后第5天停止酵母聚糖诱导。除酵母聚糖外，第3组动物腹腔注射1 g/kg/天的二甲基亚砜，第4组腹腔注射50 mg/kg/天的银杏叶提取物（EGb 761），第5组腹腔注射500 mg/kg/天的维生素C。在骨折后第7、14和21天，在乙醚麻醉下对所有动物的骨折部位进行X线摄片，以评估骨痂形成、重塑和骨桥形成情况。所有大鼠在第22天处死，对桡骨和尺骨切片进行光镜和电镜组织学及超微结构检查。采用Kruskal-Wallis方差分析检验进行统计学分析，组间比较采用Dunn多重比较检验。
单纯注射酵母聚糖的第2组出现骨愈合受损。使用各种抗氧化剂治疗的组骨愈合结果各异。第1组和第2组在组织学和放射学上骨折愈合均有极显著差异（P < 0.001）。第2组和第5组在放射学上有显著差异（P < 0.05）。
游离氧自由基对骨折愈合有负面影响，维生素C（一种抗氧化剂）可部分预防酵母聚糖对骨折愈合的负面影响。
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