Ezirganli Şeref, Kazancioğlu Hakki Oğuz, Acar Ahmet Hüseyin, Özdemir Hakan, Kuzu Emre, İnan Deniz Şahin
Program of Dental Assisting, Vocational School of Health Services, Institute of Health Science, Nişantası University, Bayrampaşa, 34030 Istanbul, Turkey.
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bezmialem Vakif University, Fatih, 34093 Istanbul, Turkey.
Exp Ther Med. 2017 May;13(5):1827-1831. doi: 10.3892/etm.2017.4166. Epub 2017 Mar 1.
Bone defects resulting from oncological surgical resections, congenital facial anomalies, trauma or infection represent a significant and common clinical problem. The present study aimed to evaluate the effects of a commercially-available medicinal plant extract product, Ankaferd BloodStopper (ABS), on bone healing. The present study was performed on 24 female ovariectomized (OVX) rats. A defect on each rat calvarium was created using a trephine burr prior to the rats being divided into two groups. Defects were grafted with a gelatin sponge soaked with normal saline (control group) or plant extract (experimental group). Half of the animals were sacrificed after 2 weeks and the others after 4 weeks. In the control group, the defects were not filled with regenerated bone. By contrast, in the experimental group, all defect areas had an increased amount of regenerated bone and connective tissue. Osteoblastic activity appeared to be greater in the experimental group however, osteoclastic activity was observed to be higher in the control group. At 2 and 4 weeks, there was a significant difference in the amount of newly regenerated bone observed in the experimental group compared with the control group (P<0.05). Therefore, the results of the present study indicated that local ABS application had a positive effect on bone healing in the OVX rat model.
肿瘤外科手术切除、先天性面部畸形、创伤或感染导致的骨缺损是一个重大且常见的临床问题。本研究旨在评估一种市售药用植物提取物产品安卡非德止血剂（ABS）对骨愈合的影响。本研究以24只雌性去卵巢（OVX）大鼠为实验对象。在将大鼠分为两组之前，先用环钻在每只大鼠的颅骨上制造一个缺损。缺损处分别植入用生理盐水浸泡的明胶海绵（对照组）或植物提取物（实验组）。一半动物在2周后处死，另一半在4周后处死。对照组的缺损处没有被再生骨填充。相比之下，实验组所有缺损区域的再生骨和结缔组织量均增加。实验组的成骨细胞活性似乎更高，然而，观察到对照组的破骨细胞活性更高。在2周和4周时，实验组新再生骨的量与对照组相比有显著差异（P<0.05）。因此，本研究结果表明，在OVX大鼠模型中局部应用ABS对骨愈合有积极作用。
