Collavo D, Colombatti A, Biasi G, Chieco-Bianchi L, Davies A J
J Natl Cancer Inst. 1976 Mar;56(3):603-8. doi: 10.1093/jnci/56.3.603.
To study the function of different lymphocyte populations in the Moloney strain of murine sarcoma virus (M-MuSV) tumorigenesis, we gave M-MuSV injections to CBA mice selectively deprived of thymus (T) lymphocytes by thymectomy, X-rradiation, and syngeneic bone marrow injection. Although no tumors appeared in the control group, 80% of the derived mice had tumors that grew progressively and ultimately killed them. In deprived mice, grafted with a syngeneic thymus (reconstituted mice) before or after an M-MuSV injection, tumors regressed or did not develop. Histologically, the lymph nodes and spleens of reconstituted mice, compared to those of deprived animals, showed repopulation of the thymus-dependent areas and prominent follicles in the cortex. Moreover, tumor tissue of reconstituted mice was extensively infiltrated by lymphocytes. To evaluate the number of lymphoid cells needed to prevent or regress M-MuSV tumors, we injected varying amounts of lymphoid cells into deprived mice. Even low lymphocyte numbers (10(6) cells) were sufficient to exert, in some cases, protection against M-MuSV tumorigenesis. This effect was not abolished by subsequent splenectomy or antilymphocyte serum treatment. Finally, deprived mice, given repeated injections of antiserum (hyperimmune) against M-MuSV, had tumors which appeared only after a prolonged latency. From these results, it is concluded that T-cell population integrity is important in affording total host protection against the M-MuSV tumors.
为研究不同淋巴细胞群体在莫洛尼鼠肉瘤病毒（M - MuSV）致瘤过程中的作用，我们对通过胸腺切除术、X射线照射及同基因骨髓注射选择性去除胸腺（T）淋巴细胞的CBA小鼠注射M - MuSV。尽管对照组未出现肿瘤，但80%的衍生小鼠发生了肿瘤，且肿瘤不断生长，最终导致小鼠死亡。在去除胸腺的小鼠中，于注射M - MuSV之前或之后移植同基因胸腺（重建小鼠），肿瘤消退或未发生。组织学检查显示，与去除胸腺的动物相比，重建小鼠的淋巴结和脾脏中胸腺依赖区重新出现细胞，皮质中有明显的滤泡。此外，重建小鼠的肿瘤组织中有大量淋巴细胞浸润。为评估预防或使M - MuSV肿瘤消退所需的淋巴细胞数量，我们向去除胸腺的小鼠注射不同数量的淋巴细胞。即使淋巴细胞数量较少（10⁶个细胞），在某些情况下也足以对M - MuSV致瘤起到保护作用。这种作用不会因随后的脾切除术或抗淋巴细胞血清治疗而消除。最后，对去除胸腺的小鼠反复注射抗M - MuSV抗血清（超免疫血清），肿瘤仅在长时间潜伏期后才出现。从这些结果可以得出结论，T细胞群体的完整性对于宿主全面抵抗M - MuSV肿瘤至关重要。
