Ponzio N M, Finke J H, Battisto J R
J Immunol. 1975 Mar;114(3):971-5.
Cells derived from lymph nodes (LN) of adult CBA mice respond blastogenically to mitomycin-treated autologous, as well as isologous spleen cells. This isogeneic LN-to-spleen (mixed lymphocyte culture) is best obtained when both responder and stimulator cells are derived from donors greater than 10 weeks of age. Responsive cells appear restricted to LN since they could not be detected in adult spleen, marrow, or thymus. LN cells do not require the presence of spleen in order to differentiate into responder cells since those derived from neonatally splenectomized mice are fully active. Stimulator cells appear in the spleen, bear Ig on their surfaces, and can be detected in spleens of irradiated, bone marrow-reconstituted mice. Experiments comparing the responsiveness of adult LN cells and that of neonatal T cells toward mitomycin C-treated lymphoid cells from a variety of sources suggest the presence of two iso-antigens on B lymphocytes. Since both antigens apparently are absent on precursor bone marrow cells and develop with time, they have been classified as murine differentiation antigens 1 and 2 (MDA-1, MDA-2). Whereas both appear in the spleen, only one, MDA-1, is also detectable by this methodology in LN. Both MDA-1 and MDA-2 activate neonatal T cells, but MDA-2 triggers only adult LN. Whereas MDA-2 developed in an x-irradiated, bone marrow-reconstituted spleen, MDA-1 did not over a 9-week interval.
源自成年CBA小鼠淋巴结（LN）的细胞对丝裂霉素处理的自体以及同基因脾细胞有增殖反应。当应答细胞和刺激细胞均来自10周龄以上的供体时，这种同基因的淋巴结-脾（混合淋巴细胞培养）反应最为明显。应答细胞似乎仅限于淋巴结，因为在成年脾脏、骨髓或胸腺中未检测到它们。淋巴结细胞分化为应答细胞不需要脾脏的存在，因为源自新生期脾切除小鼠的细胞具有完全活性。刺激细胞出现在脾脏中，其表面带有免疫球蛋白，并且可以在经照射、骨髓重建的小鼠脾脏中检测到。比较成年淋巴结细胞和新生T细胞对来自各种来源的丝裂霉素C处理的淋巴细胞的反应性的实验表明，B淋巴细胞上存在两种同种异体抗原。由于这两种抗原在前体骨髓细胞上显然都不存在，并且随时间发展，因此它们被归类为小鼠分化抗原1和2（MDA-1、MDA-2）。虽然两者都出现在脾脏中，但用这种方法在淋巴结中只能检测到其中一种，即MDA-1。MDA-1和MDA-2都能激活新生T细胞，但MDA-2仅触发成年淋巴结细胞。虽然MDA-2在经X射线照射、骨髓重建的脾脏中出现，但在9周的时间间隔内MDA-1未出现。
