Jia Kai-Zhi, Li Xiao-Hui, He Jian, Gu Li-Feng, Ma Ji-Ping, Li Shun-Peng
Key Laboratory of Agricultural Environment Microbiological Engineering, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China.
Huan Jing Ke Xue. 2007 Apr;28(4):908-12.
A monocrotophos [dimethyl (E)-1-2-methylcarbamoylvinylphosphate or MCP] -degrading strain named as M-1 was isolated from sludge collected from the wastewater treatment pool of a pesticide factory and identified as Paracoccus sp. according to its morphology and biochemical properties and 16S rDNA sequence analysis. Using MCP as a sole carbon source, M-1 was able to degrade MCP(100 mg x L(-1)) by 92.47% in 24 h. The key enzyme(s) involved in the initial biodegradation of monocrotophos in M-1 was shown to be constitutively expressed cytosolic proteins and showed the greatest activity at pH 8.0 and 25 degrees C, with its Michaelis-Mentn's constant (K(m)) and maximum degradation rate (V(max)) of 0.29 micromol x mL(-1) and 682.12 micromol (min x mg)(-1) respectively. This degrading enzyme(s) was sensitive to high temperature, but kept high activity under alkaline conditions.
从一家农药厂废水处理池收集的污泥中分离出一株名为M-1的久效磷[二甲基（E）-1-2-甲基氨基甲酰基乙烯基磷酸酯或MCP]降解菌株，根据其形态、生化特性及16S rDNA序列分析鉴定为副球菌属。以MCP作为唯一碳源，M-1能够在24小时内将100mg·L⁻¹的MCP降解92.47%。M-1中参与久效磷初始生物降解的关键酶为组成型表达的胞质蛋白，在pH 8.0和25℃时活性最高，其米氏常数（Kₘ）和最大降解速率（Vₘₐₓ）分别为0.29μmol·mL⁻¹和682.12μmol（min·mg）⁻¹。这种降解酶对高温敏感，但在碱性条件下保持高活性。
