生物膜形成菌 Paracoccus sp. 菌株 MKU1 和 MKU2 对二甲基甲酰胺的生物降解与利用。
Biodegradation and utilization of dimethylformamide by biofilm forming Paracoccus sp. strains MKU1 and MKU2.
机构信息
Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.
出版信息
Bioresour Technol. 2015;188:9-13. doi: 10.1016/j.biortech.2015.02.042. Epub 2015 Feb 16.
Two bacterial strains capable of degrading N,N-dimethylformamide (DMF) were isolated from the effluent and sludge samples of textile and tyre industries. The 16S rRNA gene analysis revealed that bacterial strains belonged to the genera Paracoccus and named as Paracoccus sp. MKU1 and Paracoccus sp. MKU2. The DMF degradation experiments conducted at a DMF concentration of 1% v/v and HPLC analysis revealed that MKU1 and MKU2 degraded 55% and 46% of DMF after 120 h of growth. Biofilm quantification by microtiter plate assay revealed that both the bacterial isolates can form efficient biofilm on during DMF utilization. The presence of secondary carbon sources influenced the DMF degradation and biofilm formation where highest biofilm formation was observed in the presence of acetate and enhanced the DMF degradation to a maximum of 86.59% with MKU1 whereas glucose and acetate enhanced DMF degradation by MKU2 to a maximum of 82.7% and 80% respectively.
从纺织和轮胎行业的废水和污泥样本中分离到两株能够降解 N,N-二甲基甲酰胺（DMF）的细菌菌株。16S rRNA 基因分析表明，这些细菌菌株属于副球菌属，分别命名为副球菌 MKU1 和副球菌 MKU2。在 1%v/v 的 DMF 浓度下进行的 DMF 降解实验和 HPLC 分析表明，MKU1 和 MKU2 在 120 小时的生长后分别降解了 55%和 46%的 DMF。微量滴定板测定法进行的生物膜定量分析表明，这两种细菌分离株在利用 DMF 时都能形成有效的生物膜。在存在次生碳源的情况下，DMF 的降解和生物膜的形成受到影响，其中在存在乙酸盐的情况下观察到最高的生物膜形成，使 MKU1 最大程度地将 DMF 降解至 86.59%，而葡萄糖和乙酸盐使 MKU2 将 DMF 降解至 82.7%和 80%。
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