Pearson Wendy, Orth Michael W, Karrow Niel A, Maclusky Neil J, Lindinger Michael I
Department Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada N1G 2W1.
Mol Nutr Food Res. 2007 Aug;51(8):1020-30. doi: 10.1002/mnfr.200700026.
New Zealand green lipped mussel (NZGLM), abalone (AB), and shark cartilage (SC) are extensively used for treatment of and/or as preventatives for arthritis, despite a relative paucity of scientific evidence for efficacy. This research integrated a simulated digestion protocol with ultrafiltration and cartilage explants to generate new information on the anti-inflammatory and chondroprotective properties of NZGLM, SC, and AB. Each nutraceutical was artificially digested using simulated gastric and intestinal fluids, and the crude digest was ultrafiltered (50 kDa). Each filtrate was applied individually to cartilage explants before the explants were stimulated with IL-1 to induce an acute inflammatory response. Media were collected daily for 48 h and analyzed for prostaglandin E(2) (PGE(2)), glycosaminoglycan (GAG), and nitric oxide (NO), and cartilage tissue was differentially stained to determine the relative proportion of live and dead cells. SC and NZGLM significantly inhibited IL-1-induced PGE(2) synthesis and IL-1-induced GAG release, and AB was an effective inhibitor of IL-1-induced NO production. The three test nutraceuticals affect at least three major pathways involved in the catabolic cycle of arthritis and may prove important treatments and/or preventatives for the pain and degradation associated with this condition. The methodology and results describe a useful model for evaluating dietary nutraceuticals in vitro.
新西兰绿唇贻贝（NZGLM）、鲍鱼（AB）和鲨鱼软骨（SC）被广泛用于治疗关节炎和/或作为关节炎的预防剂，尽管其疗效的科学证据相对较少。本研究将模拟消化方案与超滤和软骨外植体相结合，以获取有关NZGLM、SC和AB的抗炎和软骨保护特性的新信息。每种营养保健品都用模拟胃液和肠液进行人工消化，粗消化物进行超滤（50 kDa）。在软骨外植体用白细胞介素-1刺激以诱导急性炎症反应之前，将每种滤液分别应用于软骨外植体。每天收集培养基48小时，并分析前列腺素E（2）（PGE（2））、糖胺聚糖（GAG）和一氧化氮（NO），对软骨组织进行差异染色以确定活细胞和死细胞的相对比例。SC和NZGLM显著抑制白细胞介素-1诱导的PGE（2）合成和白细胞介素-1诱导的GAG释放，AB是白细胞介素-1诱导的NO产生的有效抑制剂。这三种受试营养保健品影响关节炎分解代谢周期中至少三条主要途径，可能证明是治疗和/或预防与此病症相关的疼痛和退化的重要药物。该方法和结果描述了一种在体外评估膳食营养保健品的有用模型。
