Department of Animal Biosciences, University of Guelph, Guelph, ON, N1G 2W1, Canada.
Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada.
BMC Complement Altern Med. 2019 Sep 11;19(1):252. doi: 10.1186/s12906-019-2660-z.
Turmeric is commonly used as a dietary treatment for inflammation, but few studies have evaluated the direct effect of turmeric on cartilage. The purpose of this study was to characterize cartilage explants' inflammatory responses to lipopolysaccharide in the presence of a simulated biological extract of turmeric.
Turmeric was incubated in simulated gastric and intestinal fluid, followed by inclusion of liver microsomes and NADPH. The resulting extract (TUR) was used to condition cartilage explants in the presence or absence of lipopolysaccharide. Explants were cultured for 96 h (h); the first 24 h in basal tissue culture media and the remaining 72 h in basal tissue culture media containing TUR (0, 3, 9 or 15 μg/mL). Lipopolysaccharide (0 or 5 μg/mL) was added for the final 48 H. media samples were collected immediately prior to lipopolysaccharide exposure (0 h) and then at 24 and 48 h after, and analyzed for prostaglandin E (PGE), glycosaminoglycan (GAG), and nitric oxide (NO). Explants were stained with calcein-AM for an estimate of live cells. Data were analyzed using a 2-way repeated measures (GAG, PGE, NO) or 1-way ANOVA without repeated measures (viability). Significance accepted at p < 0.05.
TUR significantly reduced PGE NO and GAG, and calcein fluorescence was reduced.
These data contribute to the growing body of evidence for the utility of turmeric as an intervention for cartilage inflammation.
姜黄通常被用作治疗炎症的饮食疗法，但很少有研究评估姜黄对软骨的直接影响。本研究的目的是描述软骨外植体在姜黄模拟生物提取物存在的情况下对脂多糖的炎症反应。
姜黄在模拟胃液和肠液中孵育，然后加入肝微粒体和 NADPH。所得提取物（TUR）用于在存在或不存在脂多糖的情况下对软骨外植体进行条件处理。外植体在组织培养基中培养 96 小时（h）；前 24 小时在基础组织培养基中，其余 72 小时在含有 TUR（0、3、9 或 15μg/ml）的基础组织培养基中。在最后 48 小时添加脂多糖（0 或 5μg/ml）。在暴露于脂多糖之前（0 小时）以及之后 24 和 48 小时立即收集培养基样本，并分析前列腺素 E（PGE）、糖胺聚糖（GAG）和一氧化氮（NO）。用 calcein-AM 对活细胞进行染色。使用 2 种方式重复测量（GAG、PGE、NO）或 1 种方式无重复测量（ANOVA）分析数据（活力）。p<0.05 时认为差异有统计学意义。
TUR 显著降低了 PGE、NO 和 GAG，calcein 荧光减少。
这些数据为姜黄作为软骨炎症干预措施的效用提供了越来越多的证据。
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