在经卡介苗治疗肿瘤已治愈的2号品系豚鼠血清中检测肿瘤细胞的补体依赖性抗体。
Detection of complement-dependent antibody to tumor cells in sera of strain-2 guinea pigs cured of their tumors by BCG Treatment.
作者信息
Boyle M D, Ohanian S H, Borsos T
出版信息
J Natl Cancer Inst. 1976 Mar;56(3):623-6. doi: 10.1093/jnci/56.3.623.
Sera of strain-2 guinea pigs (cured of line-10 tumor by BCG therapy) were tested for complement-dependent, cytotoxic antibody. About 30% of the sera tested contained significant cytotoxic acitivity with the addition of human, but not syngeneic, complement. Using papain pretreated line-10 cells, we detected antibody in about 50% of the sera with syngeneic sera as the source of complement. Antibody to line-10 was also demonstrated in selected sera by indirect fluorescence and the C1 fixation and transfer test.
对2号品系豚鼠（通过卡介苗疗法治愈10号线肿瘤）的血清进行了补体依赖性细胞毒性抗体检测。在添加人补体而非同基因补体的情况下，约30%的检测血清含有显著的细胞毒性活性。使用木瓜蛋白酶预处理的10号线细胞，我们以同基因血清作为补体来源，在约50%的血清中检测到了抗体。通过间接荧光以及C1固定和转移试验，在选定的血清中也证实了针对10号线的抗体。
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