低聚（唾液酸）的高效液相色谱法。用于测定作为共聚麦芽糖酸酶促合成中外源受体的最小链长。
H.p.l.c. of oligo(sialic acids). Application to the determination of the minimal chain length serving as exogenous acceptor in the enzymic synthesis of colominic acid.
作者信息
Ferrero M A, Luengo J M, Reglero A
机构信息
Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.
出版信息
Biochem J. 1991 Dec 15;280 ( Pt 3)(Pt 3):575-9. doi: 10.1042/bj2800575.
Abstract
A rapid, sensitive and easy h.p.l.c. method was developed for the quantitative analysis of oligosialic acids. This procedure which permits the complete separation (in 23 min) of several sialyloligomers with a degree of polymerization of between 1 and 16, has been employed to establish the minimal chain length of oligomer accepted, as an exogenous acceptor, by Escherichia coli K-235 sialytransferase complex (ST) leading to the synthesis in vitro of colominic acid. We showed that this membrane-bound enzyme catalyses the direct transfer of Neu5Ac residues (one by one) from CMP-Neu5Ac to an exogenous acceptor molecule which contains at least three Neu5Ac residues. Free Neu5Ac or (Neu5Ac)2 were not recognized as substrates, whereas the maximal rate of polymer elongation was achieved when (Neu5Ac)5 was used as substrate.
摘要
开发了一种快速、灵敏且简便的高效液相色谱法用于低聚唾液酸的定量分析。该方法能够在23分钟内将几种聚合度在1至16之间的唾液酸低聚物完全分离，已被用于确定被大肠杆菌K - 235唾液酸转移酶复合物（ST）作为外源受体接受的低聚物的最小链长，从而在体外合成结肠菌素酸。我们发现这种膜结合酶催化Neu5Ac残基（逐个）从CMP - Neu5Ac直接转移到一个至少含有三个Neu5Ac残基的外源受体分子上。游离的Neu5Ac或（Neu5Ac）₂不被识别为底物，而当使用（Neu5Ac）₅作为底物时，聚合物伸长的最大速率得以实现。
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本文引用的文献
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