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低聚（唾液酸）的高效液相色谱法。用于测定作为共聚麦芽糖酸酶促合成中外源受体的最小链长。

H.p.l.c. of oligo(sialic acids). Application to the determination of the minimal chain length serving as exogenous acceptor in the enzymic synthesis of colominic acid.

作者信息

Ferrero M A, Luengo J M, Reglero A

机构信息

Departamento de Bioquímica y Biología Molecular, Universidad de León, Spain.

出版信息

Biochem J. 1991 Dec 15;280 ( Pt 3)(Pt 3):575-9. doi: 10.1042/bj2800575.

DOI:10.1042/bj2800575
PMID:1764020
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1130494/
Abstract

A rapid, sensitive and easy h.p.l.c. method was developed for the quantitative analysis of oligosialic acids. This procedure which permits the complete separation (in 23 min) of several sialyloligomers with a degree of polymerization of between 1 and 16, has been employed to establish the minimal chain length of oligomer accepted, as an exogenous acceptor, by Escherichia coli K-235 sialytransferase complex (ST) leading to the synthesis in vitro of colominic acid. We showed that this membrane-bound enzyme catalyses the direct transfer of Neu5Ac residues (one by one) from CMP-Neu5Ac to an exogenous acceptor molecule which contains at least three Neu5Ac residues. Free Neu5Ac or (Neu5Ac)2 were not recognized as substrates, whereas the maximal rate of polymer elongation was achieved when (Neu5Ac)5 was used as substrate.

摘要

开发了一种快速、灵敏且简便的高效液相色谱法用于低聚唾液酸的定量分析。该方法能够在23分钟内将几种聚合度在1至16之间的唾液酸低聚物完全分离，已被用于确定被大肠杆菌K - 235唾液酸转移酶复合物（ST）作为外源受体接受的低聚物的最小链长，从而在体外合成结肠菌素酸。我们发现这种膜结合酶催化Neu5Ac残基（逐个）从CMP - Neu5Ac直接转移到一个至少含有三个Neu5Ac残基的外源受体分子上。游离的Neu5Ac或（Neu5Ac）₂不被识别为底物，而当使用（Neu5Ac）₅作为底物时，聚合物伸长的最大速率得以实现。

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1
H.p.l.c. of oligo(sialic acids). Application to the determination of the minimal chain length serving as exogenous acceptor in the enzymic synthesis of colominic acid.低聚（唾液酸）的高效液相色谱法。用于测定作为共聚麦芽糖酸酶促合成中外源受体的最小链长。
Biochem J. 1991 Dec 15;280 ( Pt 3)(Pt 3):575-9. doi: 10.1042/bj2800575.
2
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Successive glycosyltransfer of sialic acid by Escherichia coli K92 polysialyltransferase in elongation of oligosialic acceptors.大肠杆菌K92多唾液酸转移酶在寡唾液酸受体延伸过程中对唾液酸的连续糖基转移作用。
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引用本文的文献

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Biochemical characterization of a Neisseria meningitidis polysialyltransferase reveals novel functional motifs in bacterial sialyltransferases.脑膜炎奈瑟菌多唾液酸转移酶的生化特性揭示了细菌唾液酸转移酶中的新功能基序。
Mol Microbiol. 2007 Sep;65(5):1258-75. doi: 10.1111/j.1365-2958.2007.05862.x. Epub 2007 Jul 27.
2
Biochemical conditions for the production of polysialic acid by Pasteurella haemolytica A2.溶血巴斯德氏菌A2产生聚唾液酸的生化条件。
Glycoconj J. 1998 Sep;15(9):855-61. doi: 10.1023/a:1006902931032.
3
N-acetyl-D-neuraminic acid lyase generates the sialic acid for colominic acid biosynthesis in Escherichia coli K1.N-乙酰-D-神经氨酸裂解酶为大肠杆菌K1中结肠菌素酸的生物合成生成唾液酸。
Biochem J. 1996 Jul 1;317 ( Pt 1)(Pt 1):157-65. doi: 10.1042/bj3170157.

本文引用的文献

1
Synthesis of capsular polymers containing polysialic acid in Escherichia coli 07-K1.大肠杆菌07-K1中含多聚唾液酸的荚膜聚合物的合成
Methods Enzymol. 1982;83:540-8. doi: 10.1016/0076-6879(82)83050-4.
2
Substrate specificity of two bacteriophage-associated endo-N-acetylneuraminidases.两种噬菌体相关的内切N-乙酰神经氨酸酶的底物特异性
J Virol. 1983 Jan;45(1):367-74. doi: 10.1128/JVI.45.1.367-374.1983.
3
Cleavage of the polysialosyl units of brain glycoproteins by a bacteriophage endosialidase. Involvement of a long oligosaccharide segment in molecular interactions of polysialic acid.噬菌体唾液酸酶对脑糖蛋白多唾液酸单元的切割。长寡糖片段在多唾液酸分子相互作用中的作用。
J Biol Chem. 1985 Jan 25;260(2):1265-70.
4
Rapid separation of oligomers of polysialic acid by high-performance liquid chromatography.通过高效液相色谱法快速分离聚唾液酸寡聚物
Anal Biochem. 1987 Feb 15;161(1):181-6. doi: 10.1016/0003-2697(87)90670-1.
5
Extended polysialic acid chains (n greater than 55) in glycoproteins from human neuroblastoma cells.人神经母细胞瘤细胞糖蛋白中的延伸多唾液酸链（n大于55）
J Biol Chem. 1988 Jul 5;263(19):9443-8.
6
Polyacrylamide gel electrophoresis of the capsular polysaccharides of Escherichia coli K1 and other bacteria.大肠杆菌K1及其他细菌荚膜多糖的聚丙烯酰胺凝胶电泳
J Bacteriol. 1988 Jun;170(6):2646-53. doi: 10.1128/jb.170.6.2646-2653.1988.
7
Identification of the 38-kDa subunit of rabbit skeletal muscle glycogen synthase as glycogenin.鉴定兔骨骼肌糖原合酶的38 kDa亚基为糖原素。
Eur J Biochem. 1987 Dec 15;169(3):497-502. doi: 10.1111/j.1432-1033.1987.tb13637.x.
8
A protein-sialyl polymer complex involved in colominic acid biosynthesis. Effect of tunicamycin.一种参与结肠菌素酸生物合成的蛋白质-唾液酸聚合物复合物。衣霉素的作用
Biochem J. 1988 Apr 15;251(2):589-96. doi: 10.1042/bj2510589.
9
Glycogenin is the priming glucosyltransferase required for the initiation of glycogen biogenesis in rabbit skeletal muscle.糖原素是兔骨骼肌中糖原生物合成起始所需的引发葡糖基转移酶。
Eur J Biochem. 1988 Sep 15;176(2):391-5. doi: 10.1111/j.1432-1033.1988.tb14294.x.
10
In vitro synthesis of colominic acid by membrane-bound sialyltransferase of Escherichia coli K-235. Kinetic properties of this enzyme and inhibition by CMP and other cytidine nucleotides.大肠杆菌K-235膜结合唾液酸转移酶体外合成结肠菌素酸。该酶的动力学特性以及CMP和其他胞苷核苷酸的抑制作用。
Eur J Biochem. 1989 Jan 2;178(3):741-9. doi: 10.1111/j.1432-1033.1989.tb14505.x.