Kantrow Sara M, Boyd Alan S, Ellis Darrel L, Nanney Lillian B, Richmond Ann, Shyr Yu, Robbins Jason B
Department of Medicine (Dermatology), Vanderbilt University Nashville, TN, USA.
J Cutan Pathol. 2007 Aug;34(8):593-6. doi: 10.1111/j.1600-0560.2006.00675.x.
Activated Akt expression (p-Akt) is reportedly increased in many melanomas as compared with benign nevi. The purpose of this study was to evaluate and compare p-Akt immunohistological staining in benign nevi, Spitz nevi and primary melanomas.
Immunostaining for phosphorylated Akt was performed in 41 melanocytic lesions previously classified as benign intradermal nevus (14 lesions), Spitz nevus (9 lesions) or melanoma (18 lesions). Lesions were graded for intensity of p-Akt staining by two independent observers (0, no staining; 1, slightly positive; 2, moderately positive; 3, highly positive). Scores were averaged, and statistical analyses were performed.
Benign nevi showed less staining (mean score 1.18) compared with Spitz nevi (mean score 2.11) and melanomas (mean score 2.19). This difference was statistically significant between benign nevi and melanomas (p = 0.0047) and benign nevi and Spitz nevi (p = 0.0271). No statistical difference was detected in staining between Spitz nevi and melanomas (p = 0.8309).
Activated Akt expression is increased in Spitz nevi and melanomas as compared with benign intradermal nevi, but is unlikely to prove useful in differentiating between the former.
据报道，与良性痣相比，许多黑色素瘤中活化的Akt表达（p-Akt）增加。本研究的目的是评估和比较良性痣、Spitz痣和原发性黑色素瘤中p-Akt的免疫组织化学染色。
对41个先前分类为良性真皮内痣（14个病变）、Spitz痣（9个病变）或黑色素瘤（18个病变）的黑素细胞病变进行磷酸化Akt的免疫染色。由两名独立观察者对病变的p-Akt染色强度进行分级（0，无染色；1，弱阳性；2，中度阳性；3，强阳性）。对分数进行平均，并进行统计分析。
与Spitz痣（平均分数2.11）和黑色素瘤（平均分数2.19）相比，良性痣的染色较少（平均分数1.18）。良性痣与黑色素瘤之间（p = 0.0047）以及良性痣与Spitz痣之间（p = 0.0271）的这种差异具有统计学意义。Spitz痣和黑色素瘤之间的染色未检测到统计学差异（p = 0.8309）。
与良性真皮内痣相比，Spitz痣和黑色素瘤中活化的Akt表达增加，但不太可能有助于区分两者。
