Genovese Kenneth J, He Haiqi, Lowry Virginia K, Nisbet David J, Kogut Michael H
US Department of Agriculture, Southern Plains Agricultural Research Center, College Station, TX 77845, USA.
FEMS Immunol Med Microbiol. 2007 Oct;51(1):112-7. doi: 10.1111/j.1574-695X.2007.00286.x. Epub 2007 Jul 19.
Previous work has shown that flagellin (FGN) is a potent stimulator in vitro of phagocytic cell functions of chickens. The purpose of this study was to define the effects of FGN on the inflammatory response to Salmonella enteritidis (SE) in chickens. Intra-abdominal (IA) FGN administration caused significant increases in peripheral blood leukocytes (PBL) compared with SE-injected controls at 4 and 8 h postinjection (P<or=0.05). The heterophil (PMN) was the predominant cell responsible for the increased numbers of leukocytes in the peripheral blood. In the abdominal cavity, leukocyte infiltrates were significantly greater in FGN-injected (8 h) and SE/FGN-injected (4 and 24 h) birds than in the SE-injected control birds. Again, the predominant leukocyte infiltrating the abdominal cavity was the PMN. Bovine serum albumin (BSA)-injected protein control birds showed no increases in PBL or in abdominal cell leukocyte infiltrates over saline-injected controls. In IA challenge studies, FGN reduced SE-associated mortality (26%) compared with mortality of 51% in the SE group (P<or=0.05). BSA-injected/SE-challenged chicks had mortality similar to that of the SE group. The data suggest that FGN is a potent stimulator of a heterophil-mediated innate immune response in vivo, protecting against bacterial infections in chickens.
先前的研究表明，鞭毛蛋白（FGN）在体外是鸡吞噬细胞功能的有效刺激物。本研究的目的是确定FGN对鸡肠炎沙门氏菌（SE）炎症反应的影响。腹腔内（IA）注射FGN后，与注射SE的对照组相比，在注射后4小时和8小时外周血白细胞（PBL）显著增加（P≤0.05）。嗜异性粒细胞（PMN）是外周血白细胞数量增加的主要细胞。在腹腔中，注射FGN（8小时）和注射SE/FGN（4小时和24小时）的鸡的白细胞浸润明显多于注射SE的对照鸡。同样，浸润腹腔的主要白细胞是PMN。注射牛血清白蛋白（BSA）的蛋白对照鸡与注射生理盐水的对照鸡相比，PBL或腹腔细胞白细胞浸润没有增加。在IA攻毒研究中，与SE组51%的死亡率相比，FGN降低了SE相关死亡率（26%）（P≤0.05）。注射BSA/SE攻毒的雏鸡死亡率与SE组相似。数据表明，FGN是体内嗜异性粒细胞介导的先天性免疫反应的有效刺激物，可保护鸡免受细菌感染。
