Wang Hui-hua, Zhou Hai-yan, Chen Cong-cong, Zhang Xiu-lai, Cheng Gang
Departments of Anesthesiology and Cardiology, the Second Affiliated Hospital, Zhejiang University Medical School, Hangzhou, China.
Acta Pharmacol Sin. 2007 Aug;28(8):1175-80. doi: 10.1111/j.1745-7254.2007.00566.x.
To examine the protective effect of propofol in renal ischemia/reperfusion (I/R) injury and the role of heme oxygenase-1 (HO-1) in this process.
Sprague-Dawley rats were randomly divided into 3 groups: (i) sham-operated group; (ii) I/R group; and (iii) propofol group. Bilateral renal warm ischemia for 45 min was performed. After 2, 6, and 24 h reperfusion, blood samples and kidneys were collected for assessment of renal injury, and HO-1 expressions were analyzed by immunohistochemical analysis, RT-PCR and Western blotting.
Blood urea nitrogen and serum creatinine levels in the propofol group were significantly lower than that in the I/R group at 24 h after reperfusion. The mean histological score by Palleros standard showed that propofol significantly attenuated renal I/R injury after 6 h reperfusion. Propofol increased HO-1 mRNA and protein levels 2 h after reperfusion, whereas HO-1 expressions were present at exceedingly low levels in the I/R group and the sham-operated group at same time point. Propofol also markedly increased HO-1 mRNA and protein levels than I/R at 6 and 24 h after reperfusion.
These results suggest that propofol mitigates renal I/R injury in rats. This protection may be partly through the induction of the HO-1 expression.
探讨丙泊酚对肾缺血/再灌注（I/R）损伤的保护作用以及血红素加氧酶-1（HO-1）在此过程中的作用。
将Sprague-Dawley大鼠随机分为3组：（i）假手术组；（ii）I/R组；（iii）丙泊酚组。进行双侧肾脏45分钟的温缺血。再灌注2、6和24小时后，采集血样和肾脏以评估肾损伤，并通过免疫组织化学分析、逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法分析HO-1表达。
再灌注24小时后，丙泊酚组的血尿素氮和血清肌酐水平显著低于I/R组。根据Palleros标准得出的平均组织学评分显示，再灌注6小时后丙泊酚显著减轻了肾I/R损伤。再灌注2小时后丙泊酚增加了HO-1 mRNA和蛋白水平，而在同一时间点I/R组和假手术组中HO-1表达处于极低水平。再灌注6和24小时后，丙泊酚组的HO-1 mRNA和蛋白水平也比I/R组显著增加。
这些结果表明丙泊酚可减轻大鼠肾I/R损伤。这种保护作用可能部分是通过诱导HO-1表达实现的。
