Venkata Mohan S, Sirisha K, Sreenivasa Rao R, Sarma P N
Bioengineering and Environmental Centre, Indian Institute of Chemical Technology, Tarnaka, Hyderabad, AP 500007, India.
Ecotoxicol Environ Saf. 2007 Oct;68(2):252-62. doi: 10.1016/j.ecoenv.2007.06.002. Epub 2007 Jul 20.
Design of experimental (DOE) methodology using Taguchi orthogonal array (OA) was applied to evaluate the influence of eight biotic and abiotic factors (substrate-loading rate, slurry phase pH, slurry phase dissolved oxygen (DO), soil water ratio, temperature, soil microflora load, application of bioaugmentation and humic substance concentration) on the soil bound chlorpyrifos bioremediation in bioslurry phase reactor. The selected eight factors were considered at three levels (18 experiments) in the experimental design. Substrate-loading rate showed significant influence on the bioremediation process among the selected factors. Derived optimum operating conditions obtained by the methodology showed enhanced chlorpyrifos degradation from 1479.99 to 2458.33microg/g (over all 39.82% enhancement). The proposed method facilitated systematic mathematical approach to understand the complex bioremediation process and the optimization of near optimum design parameters, only with a few well-defined experimental sets.
运用田口正交阵列（OA）的实验设计（DOE）方法，用于评估八个生物和非生物因素（底物加载速率、浆液相pH值、浆液相溶解氧（DO）、土壤水比例、温度、土壤微生物负荷、生物强化应用和腐殖质浓度）对生物泥浆相反应器中土壤结合毒死蜱生物修复的影响。在实验设计中，所选的八个因素在三个水平上进行考虑（18个实验）。在所选因素中，底物加载速率对生物修复过程显示出显著影响。通过该方法获得的最佳操作条件表明，毒死蜱降解率从1479.99微克/克提高到2458.33微克/克（总体提高了39.82%）。所提出的方法促进了系统的数学方法，以理解复杂的生物修复过程并优化接近最佳的设计参数，仅需少量明确的实验组。
