Gheorghe Emma, Tomuţa V, Mehedinţi T, Hîncu Mihaela, Broască V
Histology Department, Faculty of Medicine, Ovidius University, 58 Ion Vodă Street, Constanţa, Romania.
Rom J Morphol Embryol. 2007;48(2):151-4.
The present study puts forth the morphometric analysis of modifications that occur in the ovarian structures (stroma, parenchyma, blood vessels) before and after menopause onset. Our study was performed on 30 human ovary specimens from patients with no history of genital pathology that were split in two groups (15 ovaries harvested from fertile women and 15 ovaries harvested from women at menopause). The specimens were processed following the classic histological technique of paraffin embedding and stained using Hematoxylin-Eosin technique and trichromic Goldner-Szeckelly technique. The samples were examined and assessed using Nikon E-600 light microscope. The images captured by computer were processed using LUCIA G 4.10 software. To evaluate the histological modifications, we chose the following parameters: Area Fraction of blood vessels and Circularity of blood vessels. The study shows that modifications of the ovary components are synchronized. The vascular changes that occur after menopause onset lead to a poor vascular supply of all ovarian components: germinal epithelium, ovarian follicles and ovarian stroma. The comparative morphometric analysis on premenopause and postmenopause ovarian specimens sustains the idea that the ovarian involution starts from the ovarian vessels, which are the first structures affected by hyaline degeneration.
本研究对绝经前后卵巢结构（基质、实质、血管）发生的改变进行了形态计量学分析。我们的研究对30例无生殖系统疾病史的人类卵巢标本进行，这些标本被分为两组（15例取自育龄妇女的卵巢，15例取自绝经后妇女的卵巢）。标本按照石蜡包埋的经典组织学技术进行处理，并用苏木精-伊红技术和三色Goldner-Szeckelly技术进行染色。使用尼康E-600光学显微镜对样本进行检查和评估。通过计算机捕获的图像使用LUCIA G 4.10软件进行处理。为了评估组织学改变，我们选择了以下参数：血管面积分数和血管圆形度。研究表明，卵巢各组成部分的改变是同步的。绝经后发生的血管变化导致所有卵巢组成部分（生发上皮、卵巢卵泡和卵巢基质）的血液供应不足。绝经前和绝经后卵巢标本的比较形态计量学分析支持了这样一种观点，即卵巢退化始于卵巢血管，卵巢血管是受透明变性影响的首批结构。
