采用放射性BACTEC法和酶促吡嗪酰胺酶法对结核分枝杆菌的吡嗪酰胺药物敏感性进行比较。
Comparison of pyrazinamide drug susceptibility of M. tuberculosis by radiometric BACTEC and enzymatic pyrazinamidase assay.
作者信息
Krishnamurthy A, Almeida D, Rodrigues C, Mehta A
机构信息
Department of Microbiology, P.D Hinduja National Hospital and Medical Research Centre, Mumbai - 400 016, India.
出版信息
Indian J Med Microbiol. 2004 Jul-Sep;22(3):166-8.
The aim of this study was to perform pyrazinamide (PZA) susceptibility testing of M. tuberculosis by enzymatic PZA assay and compare the results with radiometric BACTEC 460 TB system and LJ proportion method. One hundred and thirty clinical isolates of M. tuberculosis were included in the study. Of the 130 clinical isolates tested, five were resistant and 124 were sensitive by both methods thus giving overall sensitivity and specificity of 83.33% and 100% respectively. Concordance was found in 129 out of 130 strains tested by all three methods. Pyrazinamidase assay can be used as an alternative to BACTEC and LJ proportion method.
本研究的目的是通过酶法吡嗪酰胺（PZA）检测对结核分枝杆菌进行PZA药敏试验，并将结果与放射性BACTEC 460 TB系统和罗氏比例法进行比较。该研究纳入了130株结核分枝杆菌临床分离株。在检测的130株临床分离株中，两种方法均显示5株耐药，124株敏感，因此总体敏感性和特异性分别为83.33%和100%。在130株检测菌株中，有129株三种方法检测结果一致。吡嗪酰胺酶检测可作为BACTEC法和罗氏比例法的替代方法。
Zotero 插件